| Project/Area Number |
14560263
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Tokyo University Agriculture And Technology |
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Principal Investigator |
HAYASHIDANI Hideki Tokyo University Agriculture And Technology, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (30180988)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Yersinia enterocolitica / Yersinia pseudotuberculosis / wild rodent / Infection / Habitat isolation / LAMP / Japan / Yersinia.enterocolitica / Yersinia.pseudotsuberculosis |
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| Research Abstract |
Habitat isolation of pathogenic Yesinia has been observed in wild rodents, such as large Japanese field mouse, living in Japan. The large Japanese field mouse living in east area of Honshu Island harbore Y enterocolitica serovar O:8, On the other hand, the rodents living in west area of Honshu Island harbor Y pseudotuberculosis This study was conducted to clarify the mechanism of habitat isolation of pathogenic Yersinia observed in wild rodent living in Japan.In addition, loop-mediated isothermal amplification (LAMP) method was evaluated to detect Y pseudotuberculosis from wild rodents. Results obtained were as follow.
1)Large Japanese field mice originated from east or west area of Honshu Island were challenged orally with Y enterocolitica serovar O:8 or Y pseudotuberculosis. Both mice originated from east or west area of Honshu Island were infected with Y enterocolitica serovar O:8 and Y pseudotuberculosis,and.shed the bacteria for 21-28 days after challenge. Since both mice originated from east or west area of Honshu Island showed susceptibility against Y enterocolitica serovar O:8 and Y pseudotuberculosis, we were not able to clarify the mechanism of habitat isolation of pathogenic Yersinia observed in wild rodent living in Japan.
2) We developed a Loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 minutes by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y pseudotuberculosis.
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