| Project/Area Number |
14560278
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | National Agriculture and Bio-oriented Research Organization |
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Principal Investigator |
SHIONO Hiroki National Agriculture and Bio-oriented Research Organization, Clinical Biochemistry Section, Hokkaido Research Station, National Institute of Animal Health, Chief, 動物衛生研究所・北海道支所・臨床生化学研究室, 室長 (30343958)
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| Co-Investigator(Kenkyū-buntansha) |
YAGI Yukio National Agriculture and Bio-oriented Research Organization, Research Planning Section, Department of Planning and Coordination, National Institute of Animal Health, Chief, 動物衛生研究所・企画調整部・研究企画科, 科長
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | bovine theileriosis / anemia / red blood cells / oxidation / autoantibody / phosphatidylserine / Heinz body / erythronhagocytosis / 牛 / 貪食 |
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| Research Abstract |
The primary clinical symptom of Japanese bovine theileriosis, caused by the intraerythrocytic protozoan Theileria orientails, is anemia, but the underlying pathogenesis of the anemia still unknown. This study was carried out to clarify the mechanism of this anemia's development from the viewpoint of oxidative stress to red blood cells (RBC) exerted by reactive oxygen species.
The new findings of this study are as follows : (1) during the progression of anemia in T. orientalis-infected cattle, the binding of immunoglobulin G (IgG) antibody to RBC showed a proportionate increase with the development of anemia; (2) binding of IgG in infected serum to oxidant-treated RBC was higher than that to untreated RBC;
(3) phosphatidylserine (PS) expression on the surface of RBC in infected cattle began to increase markedly during the onset of anemia : the oxidant-treated RBC were compared with untreated RBC of the same infected individual, with remarkable appearance of PS on the surface of oxidant-treated RBC; (4) during the onset of anemia in T. orlentalis-infected cattle, peroxide levels, as indicator of intraerythrocytic oxidation, increased markedly with the development of anemia; (5) during the progression of anemia in T. orientalis-infected cattle, the levels of Heinz body formation increased in proportion with the development of anemia ; and (6) macrophages obtained from peripheral blood of infected cattle exhibited increased erythrophagocytic activity compared to those from non-infected cattle.
In this study, it was revealed from the biochemical analysis of RBC that oxidative stress, i.e., an increase in oxidative damage to RBC, plays an important role in the development of anemia in Japanese bovine theileriosis.
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