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Morphological characterization of the cells present in niches of the normal and reconstructed epithelia

Research Project

Project/Area Number14570006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionIwate Medical University(2003)
Niigata University(2002)

Principal Investigator

HITOMI Jiro  Iwate Medical University, School of Medicine, Professor (00218728)

Co-Investigator(Kenkyū-buntansha) HIRANO Sigeki  Niigata University, School of Medicine, Professor (10018765)
Project Period (FY) 2002 – 2003
Project Status Completed(Fiscal Year 2003)
Budget Amount *help
¥4,100,000 (Direct Cost : ¥4,100,000)
Fiscal Year 2003 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Fiscal Year 2002 : ¥2,900,000 (Direct Cost : ¥2,900,000)
Keywordsepithelial niche / stem cells / monoclonal antibodies / immunohistochmistry / tissue engineering / skin / hair follicle / esophagus / 食道上皮 / 肝細胞 / 血管 / 腺
Research Abstract

We attempted to produce novel monoclonal antibodies recognizing the niche cells of the human esophageal epithelium. Hybridomas were generated from fusions between spleen cells of ddY mice immunized with esophageal epithelial cells (EECs) cultured at low calcium concentrations. A clone, NJ-E-F4 was selected for its staining pattern on frozen sections of the epithelia, which produces a monoclonal antibody (moAb) reacting with almost cells of human esophageal epithelia, except the small populations of non-proliferating cells in the basal layer. The immunoreactivities of NJ-E-F4 were detected at Golgi area of cytoplasm in the cells predominantly located in the basal to the third layer. Ki67 positive proliferating cells ware observed in the second layer just above F4 negative cells in the basal layer, which were positive for our previous established NJ-E-H10 moAb. H10 moAb recognizes the basal cells in the interpapillary zones of the epithelia, and some of which seem to be the candidates of the stem cell in esophageal epithelia. The data seemed to be indicated that F4 negative, H10 positive and Ki67 negative cells are the candidates for the epithelial stem cells, which migrate into the second layer and start the cell cycle positive for Ki67. On the other hand, H10 positive cells in the rat skin were also observed in the hair follicle bulge which is the epidermis niche. So we used rat fetal H10 positive epithelia for the graft to grow the skin with hair follicles on the chorion of chick embryo. We succeeded to make the epidermis without differences of the H10 positive cells population and location between in the reconstructed and the normal. Therefore our moAb H10 and F4 are possibly to be good tools for the morphological examination of the epithelial niche cells.

Report

(3results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

Research Products

(8results)

All 2005 2003 Other

All Journal Article Presentation Publications

  • [Journal Article] ニワトリ胚漿尿摸上でラットの上皮組織を再構成する2003

    • Author(s)
      人見 次郎, 平野 茂樹
    • Journal Title

      解剖学雑誌 78

      Pages : 199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Bclllb is required for differentiation and survival of αβ T lymphocytes2003

    • Author(s)
      Wakabayashi Y, Watanabe H, Inoue J, Takeda N, Sakata J, Mis hima Y, Hitomi J, Ymamamoto T, Utsuyama M, Niwa O, Aizaw a S, Kominami R.
    • Journal Title

      Nat Immunol 4

      Pages : 533-539

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development and characteristics of hair follicles and other skin appendages in the graft of rat fetal epithelia on the chorion of chick embryo2003

    • Author(s)
      Hitomi, J., Hirano, S
    • Journal Title

      Acta Anatomica Nipponia 78 Supplment 199

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Bcl11b is required for differentiation and survival ofαβT lymphocytes2003

    • Author(s)
      Wakabayashi, Y., Watanabe, H., Inoue, J., Takeda, N., Sakata, J., Mishima, Y., Hitomi, J., Ymamamoto, T., Utsuyama, M., Niwa, O., Aizawa, S., Kominami, R
    • Journal Title

      Nat Immunol 4

      Pages : 533-539

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Presentation] 上皮内niche(幹細胞成育微小環境)の形態学的解析と再構成系確立への試み2005

    • Author(s)
      人見 次郎
    • Organizer
      第69回日本皮膚科学会、東部支部総会学術大会
    • Place of Presentation
      盛岡
    • Year and Date
      2005-09-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Presentation] Development and characteristics of hair follicles and other skin appendages in the graft of rat fetal epithelia on the chorion of chick embryo2003

    • Author(s)
      Hitomi, J., Hirano, S
    • Organizer
      The 108th Annual meeting of the Japanese Association of Anatomists
    • Place of Presentation
      Fukuoka, JAN
    • Year and Date
      20030400
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Presentation] ニワトリ胚漿尿膜上でラットの上皮組織を再構成する2003

    • Author(s)
      人見 次郎, 平野 茂樹
    • Organizer
      第108回日本解剖学会総会、全国学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2003-04-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 人見次郎, 平野茂樹: "ニワトリ胚漿尿膜上でラットの上皮組織を再構成する"解剖学雑誌. 78巻(抄録号). 199 (2003)

    • Related Report
      2003 Annual Research Report

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Published : 2002-04-01   Modified : 2016-04-21  

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