|Budget Amount *help
¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 2003 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 2002 : ¥2,400,000 (Direct Cost : ¥2,400,000)
The relationship with stromal cells and follicular dendritic cells, which are considered to influence the structure of lymph node and the adjustment of microenvironment, was investigated used by IL-2 receptorγ chain knockout mouse.
We had observed the ultrastructure by the electron microscopy. And the peculiar characteristic for the cell was detected by using monoclonal antibody, that is MOMA-1,MOMA-2,TCR-β chain,CD45R,MIDC-8,FDC-M1 and FDC-M2, for immuno histochemistry.
1.The IL-2 Rγ chain did not lead the decrease the numbers of TcRβ(+) T cells, but decrease CD45R(+) B cells in the lymph node and spleen. It dose not influence the MIDC-8 positive dendritic cells which were located in the T cell area even if the structure of lymph node is not complete. The numbers of monocyte were increased, but MOMA-1(+) cells in spleen were disappeared, but much ones in lymph node. Precursor of FDCs was included in a group of stromal cells in this mouse. Because after the stimulation by the antigen, there was observed typical FDC elongated cytoplasmic processes with antigens between lymphocytes. The B cell did not play critical role to differentiate from immature FDC to mature.
2.The stromal cells separated from IL-2R KO mouse were cultured with memory T cell, memory B cell and immune complexes. When we used the method of the separation FDC from immature lymph node which is difficult to separate from T cell areas and B cell area, we could get the precursor cells
Our result suggested that IL-2 receptorγ chain may do not play critical roles on the differentiation of FDC. This model is very useful for investigation of functional construction and microenvironment of stromal cells and FDC.