| Project/Area Number |
14570014
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | HIROSHIMA UNIVERSITY |
|---|
Principal Investigator |
SUZAKI Etsuko Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (10274052)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KATAOKA Katsuko Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30034002)
SAITO Akira Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (80346486)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
|
|---|
| Keywords | gamma-tubulin / microtubule / cell adhesion molecule / cells-meeting junction / three-dimensional analysis / proteome analysis / NOXO 1 / immunoelectron microscopy / NOXO1 / 3細胞ジャンクション / 免疫蛍光染色 |
|---|
| Research Abstract |
Microtubules play important roles in maintaining the cell morphology and polarity. We had been studying to clarify the localization of gamma-tubulin in a polarized cell which organizes microtubule arrays and found that one of anti-gamma-tubulin antibody, G9, stained three-cells meeting junctions in the mouse duodenal enterocyte. Therefore, we have investigated the precise localization of G9-staining molecule and its molecular property for the two years of 2002-2003
G9 staining was examined by using a confocal microscope, and it was shown that G9 stained three-cells-junction in the middle to upper part of lateral membranes. Especially, the staining of the top regions were strong but was totally different from the one of E-cadherin. Immunoelectronmicroscopy was further performed, and the labels of an anti-gamma-tubulin antibody and G9 were seen around the three-cells-meeting junctions where the labels of an anti-alfa-tubulin antibody were also detected. These results confirmed that a gamma-tubulin-like molecule localizes at three-cells-meeting junction associating with microtubules
In order to clarify G9-recognising molecule, the proteome analysis and succeeding peptide-MASS-fingerprint analysis was also performed. The proteome analysis demonstrated that there was one candidate which would be valuable for the further analysis. The peptide-MASS-fingreprint analysis suggests that the G9-recognizing molecule is possibly NOXO (NASPH-oxidase organizer) 1 whose c-DNA was newly identified in 2003. Further investigations will be needed to work out more details
|
