A basic study for mitochondria-targeting liposome vector using human mitochondrial fusiogenic protein.

• Project/Area Number: 14570129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Jichi Medical University
• Principal Investigator: ENDO Hitoshi Jichi Medical School, Department of Biochemistry, Associate Professor, 医学部, 助教授 (50221817)
• Project Period (FY): 2002 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: mitochondria / liposome / gene therapy / proteoliposome / membrane fusion / mitochondrial morphogenesis / mitochondrial membarne / GTP binding protein

Research Abstract

The mitochondria are organelle in the cell and have the proper DNA different from the nuclear genome. The development of the new way of introducing a material into the mitochondria, which is different from the usual gene therapy to the nuclear target, is necessary for the fundamental treatment of the mitochondrial disease based on the mitochondrial DNA. In this research, it aims at the fundamental development of new liposome vector that peculiarly targets the mitochondria, using the mitochondrial fusiogenc factor as an effective component. Such a vector will make it possible that not only normal mitochondrial DNA but also the drug adjusting the mitochondrial function and/or the exogenous ribozyme regulating the mitochondrial transcripts are introduced into the mitochondria. The development of such a vector would become applied for a new drug delivery system for many diseases with dysfunction of the mitochondria in the future.
We identified a mitochondrial fusiogenic protein, a human Fzo homolog, and analyzed the domain of the protein necessary for membrane fusiogenic activity. We also produced the recombinant protein of the domain, reconstituted the proteoliposome using the protein, and proved the membrane fusiogenic activity of the liposome in vitro. On the other hand, we examined the OPA1 protein which has the mitochondrial fusiogenic activity, and showed the differential sublocalization of OPA1 isoform in the human cell. This factor is one of the assistant factors of the mitochondrial fusiogenic device, which will be important for improvement for the efficiency of the liposome vector. Next step requires an examination whether the mitochondrial DNA-embedded liposome, introduced in the cell by microinjection, actually targets the mitochondria in vivo. This research is the first step to establish the fundamental technology of the gene therapy of mitochondrial diseases.

Research Products

• [Journal Article] Activation of Pre-mRNA Splicing by Human RNPS1 Is Regulated by CK2 Phosphorylation. (2005)
  • Author(s): Trembley, J.H.
  • Journal Title: Mol.Cell.Biol. 25 Pages: 1446-1457
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Activation of Pre-mRNA Splicing by Human RNPS1 Is Regulated by CK2 Phosphorylation. (2005)
  • Author(s): Trembley, J.H., Tatsumi, S., Sakashita, E., Loyer, P., Slaughter, C.A., Suzuki, H., Endo, H., Kidd, V.J., Mayeda, A.
  • Journal Title: Mol.Cell.Biol. 25 Pages: 1446-1457
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Damaged DNA-binding protein 2 accelerates UV- damaged DNA repair in human corneal endothelium. (2004)
  • Author(s): Inoki, T.
  • Journal Title: Exp.Eye Res. 79(3) Pages: 367-376
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of Cre/LoxP recombination system in transgenic rats. (2004)
  • Author(s): Sato, Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 319 Pages: 1197-1202
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human RNPS1 and Its associated factors : a versatile alternative pre-mRNA splicing regulator in vivo. (2004)
  • Author(s): Sakashita, E.
  • Journal Title: Mol.Cell.Biol. 24 Pages: 1174-1187
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human DDB2 splicing variants are dominant negative inhibitors of UV-damaged DNA repair. (2004)
  • Author(s): Inoki, T.
  • Journal Title: Biochem.Biophys.Res.Commun. 314 Pages: 1036-1043
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human DDB2 splicing variants are dominant negative inhibitors of UV-damaged DNA repair. (2004)
  • Author(s): Inoki, T., Yamagami, S., Inoki, Y., Tsuru, T., Hamamoto, T., Kagawa, Y., Mori, T., Endo, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 314 Pages: 1036-1043
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human RNPS1 and Its associated factors : a versatile alternative pre-mRNA splicing regulator in vivo. (2004)
  • Author(s): Sakashita, E., Tatsumi, S., Werner, D., Endo, H., Mayeda, A.
  • Journal Title: Mol.Cell.Biol. 24 Pages: 1174-1187
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Establishment of Cre/LoxP recombination system in transgenic rats. (2004)
  • Author(s): Sato, Y., Endo, H., Ajiki, T., Hakamata, Y., Okada, T., Murakami, T., Kobayashi, E.
  • Journal Title: Biochem.Biophys.Res.Commun. 319 Pages: 1197-1202
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Damaged DNA-binding protein 2 accelerates UV-damaged DNA repair in human corneal endothelium. (2004)
  • Author(s): Inoki, T., Endo, H., Inoki, Y., Hamamoto, T., Tsuru, T., Mori, T., Miyata, K., Amano, S., Yamagami, S.
  • Journal Title: Exp.Eye Res. 79(3) Pages: 367-376
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A member of Forkhead transcription factor FKHRL1 is a downstream effector of STI571-induced cell cycle arrest in BCR-ABL expressing cells (2003)
  • Author(s): Komatsu, N., Watanabe, T., Uchida, M., Mori, M., Kirito, K., Kikuchi, S., Liu, Q., Tauchi, T., Miyazawa, K., Endo, H., Nagai, T., Ozawa, K.
  • Journal Title: J.Biol.Chem. 278 Pages: 6411-6419
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential sublocalization of Dynamin-related protein OPA1 isoforms in mitochondria. (2003)
  • Author(s): Satoh, M., Hamamoto, T., Seo, N., Kagawa, Y., Endo, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 300 Pages: 482-493
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Stage-specific regulatory element of mouse Sry gene. (2003)
  • Author(s): Yokouchi, K., Ito, M., Nishino, K., Yamanouchi, K., Naito, K., Suzawa, M., Kato, S., Hakamata, Y., Endo, H., Tojo H.
  • Journal Title: Mol.Reprod.Dev. 64 Pages: 389-396
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Defective sorting to secretory vesicles in trans-Golgi network is partly responsible for protein C deficiency. Molecular mechanisms of impaired secretion of abnormal protein C R169W, R352W, and G376D. (2003)
  • Author(s): Naito, M., Mimuro, J., Endo, H., Madoiwa, S., Ogata, K.-I., Kikuchi, J., Sugo, T., Yasu, T., Kariya, Y., Hoshino, Y., Sakata, Y.
  • Journal Title: Circ.Res. 92 Pages: 865-872
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG. (2003)
  • Author(s): Jin, Y., Suzuki, H., Maegawa, S., Endo, H., Sugano, S., Hashimoto, K., Yasuda, K., Inoue, K.
  • Journal Title: EMBO J. 22 Pages: 905-912
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polymorphism, heteroplasmy, mitochondrial fusion and diabetes. (2003)
  • Author(s): Sato, A., Endo, H., Umetsu, K., Sone, H., Yanagisawa, Saigusa, A., Aita, S., Kagawa, Y.
  • Journal Title: Bioscience Rep. 23 Pages: 313-337
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polymorphism, heteroplasmy, mitochondrial fusion and diabetes. (2003)
  • Author(s): Sato, A.
  • Journal Title: Bioscience Rep. 23 Pages: 313-337
• [Journal Article] Muscle-specific exonic splicing silencer for exon exclusion in human ATP synthase gamma-subunit pre-mRNA. (2002)
  • Author(s): Hayakawa, M., Sakashita, E., Ueno, E., Tominaga, S., Hamamoto, T., Kagawa, Y., Endo, H.
  • Journal Title: J.Biol.Chem. 277 Pages: 6974-6984
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isoliquiritigenin suppresses pulmonary metastasis of mouse renal cell carcinoma. (2002)
  • Author(s): Yamazaki, S., Morita, T., Endo, H., Hamamoto, T., Baba, M., Joichi, Y., Kaneko, S., Okada, Y., Okuyama, T., Nishino, H., Tokue, A.
  • Journal Title: Cancer Lett. 183 Pages: 23-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Apaf-1 is a mediator of E2F-1-induced apoptosis. (2002)
  • Author(s): Furukawa, Y., Nishimura, N., Furukawa, Y., Satoh, M., Endo, H., Iwase, S., Yamada, H., Matsuda, M., Kano, Y., Nakamura, M.
  • Journal Title: J.Biol.Chem. 277 Pages: 39760-39768
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] cDNA cloning and characterization of Drb1, a new member of RRM-type neural RNA-binding protein. (2002)
  • Author(s): Tamada, H., Sakashita, E., Shimazaki, K., Ueno, E., Hamamoto, T., Kagawa, Y., Endo, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 297 Pages: 96-104
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vitro Cre-loxP system in cells from developing gonads : Investigation of the Sry promoter. (2002)
  • Author(s): Ito, M., Yokouchi, K., Naito, K., Endo, H., Hakamata, Y., Miyazaki, J.-I., Tojo H.
  • Journal Title: Dev.Growth Differ. 44 Pages: 549-557
  • NAID: 50000720752
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Inoki, T.: "Human DDB2 splicing variants are dominant negative inhibitors of UV-damaged DNA repair."Biochem.Biophys.Res.Commun.. 314. 1036-1043 (2004)
• [Publications] Sakashita, E.: "Human RNPS1 and Its associated factors : a versatile alternative pre-mRNA splicing regulator in vivo."Mol.Cell.Biol.. 24. 1174-1187 (2004)
• [Publications] Naito, M.: "Defective sorting to secretory vesicles in trans-Golgi network is partly responsible for protein C deficiency."Circ.Res.. 92. 865-872 (2003)
• [Publications] Yokouchi, K.: "Stage-specific regulatory element of mouse Sry gene."Mol.Reprod.Dev.. 64. 389-396 (2003)
• [Publications] Jin, Y.: "A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG."EMBO J.. 22. 905-912 (2003)
• [Publications] Satoh, M.: "Differential sublocalization of Dynamin-related protein OPA1 isoforms in mitochondria."Biochem.Biophys.Res.Commun.. 300. 482-493 (2003)
• [Publications] 遠藤仁司: "分子生物学イラストレイテッド 改訂第2版"田村隆明、山本 雅 編集、羊土社. 380 (2003)
• [Publications] Jin, Y.: "A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG"EMBO J. 22. 905-912 (2003)
• [Publications] Satoh, M.: "Differential sublocalization of the dynamin-related protein DPA1 isoforms in mitochondria"Biochem.Biophys.Res.Commun.. 300. 482-493 (2003)
• [Publications] Tamada, H.: "cDNA cloning and characterization of Drb1, a new member of RRM-type neural RNA-binding protein"Biochem.Biophys.Res.Commun.. 297. 96-104 (2002)
• [Publications] Furukawa, Y.: "Apaf-1 is a mediator of E2F-1-induced apoptosis"J.Biol.Chem.. 277. 39760-39768 (2002)
• [Publications] Hayakawa, M.: "Muscle-specific exonic splicing silencer for exon exclusion in human ATP synthase γ-subunit pre-mRNA"J.Biol.Chem.. 277. 6974-6984 (2002)
• [Publications] Kirito, K.: "TPO regulates Bcl-xL gene expression through Stat5 and phosphatidylinositol-3-kinase activation pathways"J.Biol.Chem.. 277. 8329-8337 (2002)
• [Publications] 遠藤 仁司: "分子生物学 イラストレイテッド 改訂第2版"田村隆明,山本雅編 羊士社. 380 (2003)