|Budget Amount *help
¥3,400,000 (Direct Cost : ¥3,400,000)
Fiscal Year 2003 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 2002 : ¥1,800,000 (Direct Cost : ¥1,800,000)
Although it is now accepted that there are correlations between human papillomavirus (HPV) types and specificcytopathic changes (HPV type-specific cytopathic effect : CPE), the true mechanism of the CPE is still unknown. The cytopathic effects of HPV 1, the related HPV types (HPV 4, 60 and 65), and HPV 63 are intracytoplasmic granular (Gr-), homogeneous (Hg-) and filamentous (Fl-) inclusions (ICBs), respectively. Because of its connection with tonofilaments, HPV 1-associated Gr-ICBs have been identified as abnormally developed keratohyaline granules. In this study, we attempted comparative microscopic and electron microscopic analyses between the granular, homogeneous and filamentous inclusions.
Microscopically, Gr-ICBs were many eosinophilic granules appeared in each cell of the epidermal cells, Hg-ICBs were the homogeneous, eosinophilic, intracytoplasmic substance appeared as a single inclusion in each cell, and Fl-ICBs were a baso-eosinophilic intracytoplasmic substance which contained filamentous structure, respectively. Electron-microscopically, Gr-ICBs varied in shape and consisted of aggregated tonofilament-like fibrillar structures : Hg-ICBs consisted of aggregated granular structures : and Fl-ICBs were composed of a mixture of tonofibril-like thick fibrillar structures and keratohyaline-like granular structures. Our preliminary studies have revealed the specific association of a protein coded for E4 gene of each HPV type with the different inclusion bodies.