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Molecular biological analysis of the genes related to viral oncogenesis

Research Project

Project/Area Number 14570266
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNagasaki University

Principal Investigator

MORIUCHI Ryozo  Nagasaki University, Graduate School of Biomedical Sciences, Department of Molecular Microbiology & immunology, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (60210142)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsoncogene / T get / ATL / RECK / Invasion activity / NF-κB / 成人T細胞白血病 / がん関連遺伝子 / RhoGEF / トランスフォーメーション / Tax
Research Abstract

We screened for ATL tumor cell-derived cDNAs with the potential to transform murine fibroblasts, NIH3T3, and succeeded in cloning a trio related transforming gene in ATL tumor cells (Tgat). Tgat was expressed exclusively in leukemic cells of acute or chronic ATL patients and cell lines originating from ATL cells, but the expression could not be detected in PBMCs of healthy volunteers, healthy HTLV-1 carriers, or HTLV-1 infected cell lines established in vitro. Comparison of the cDNA with the human. genome database revealed that Tgat is alternatively transcribed from the Trio-encoding gene (from exons 38 to 46) and spliced to a novel exon located downstream of exon 58. We found that both the Rho-GEF activity and the C-terminal region of Tgat were required for not only transforming but also invasion-inducing activity. These results indicate that Tgat expression contributes to the malignant profiles of ATL tumor cells.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 森内 良三: "ATL細胞に発現する新規がん関連遺伝子Tgatの機能"血液フロンティア(特集:ATL〜最近の進歩〜). 14・1. 21-29 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tawara M.et al.: "Aberrant processing of Fas transcripts in adult T-cell leukemia : a possible role in tumor cell survival"Cncer Letter. 193・2. 235-242 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita F.et al.: "Inactivation of porcine endogenous retrovirus by human serum as a function of complement activated through the classical pathway"Hepatology Researh. 26・2. 106-113 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森内 良三: "ATL細胞特異的ながん関連遺伝子"レトロウイルス研究の最前線. 151-160 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tawara M., Maeda T., Yamada Y., Harasawa H., Tsuruda K., Sugahara K., Moriuchi R., Tomonaga M., Kamihira S.: "Aberrant processing of Fas transcripts in adult T -cell leukemia : a possible role in tumor cell survival."Cancer Lett.. 193(2). 235-242 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujita F., Yamashita-Futsuki I., Eguchi S., Kamohara Y., Fujioka H., Yar aga K., Furui J., Moriuchi R., Kanematsu T., Katamine S.: "Inactivation of porcine endogenous retrovirus by human serum as -a function of complement activated through the classical pathway."Hepatol Res.. 26(2). 106-113 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 森内 良三: "ATL細胞に発現する新規がん関連遺伝子Tgatの機能"血液フロンティア(特集:ATL〜最近の進歩〜). 14・1. 21-29 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tawara M.et al.: "Aberrant processing of Fas transcripts in adult T-cell leukemia : a possible role in tumor cell survival"Cancer Letter. 193・2. 235-242 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujita F.et al.: "Inactivation of porcine endogenous retrovirus by human serum as a function of complement activated through the classical pathway"Hepatology Researh. 26・2. 106-113 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森内 良三: "ATL細胞特異的ながん関連遺伝子"レトロウイルス研究の最前線. 151-160 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tawara M. et al.: "Aberrant processing of Fas transcripts n adult T-cell leukemia : a possible role in tumor cell survival"Cancer Letter. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Okazaki S. et al.: "HTLV-1 Proviruses Encoding Non-Functional TAX in Adult T-Cell Leukemia"Virus Gene. 23(2). 123-135 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kohno T. et al.: "Identification of Genes Associated with the Progression of Adult T Cell Leukemia (ATL)"Jpn J Cancer Res. 91. 1103-1110 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakane S. et al.: "Comparative molecular analysis of HTLV-I proviral DNA in HTLV-I infected members of a family with a discordant HTLV-I-associated myelopathy in monozygotic twins"J Neurovirol. 6. 275-283 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seki M.et al.: "Up-regulation of human T lymphotropic virus type 1 (HTLV-1) tax/rex mRNA in infected lung tissues"Cli Exp Immunol. 120. 488-498 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Maeda T. et al.: "Fas gene mutation in the progression of adult T cell leukemia"J Exp Med. 189. 1063-1071 (1999)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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