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Studies on species identification and evolution using p53 gene analysis.

Research Project

Project/Area Number14570391
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionSapporo Medical University

Principal Investigator

AZUMI Jun-ichi  Sapporo Medical University, Medicine, Assistant Professor, 医学部, 講師 (00045551)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Hiroshi  Sapporo Medical University, Medicine, Professor, 医学部, 教授 (60263092)
TAMAKI Keiji  Kyoto University, Medicine, Professor, 医学部, 教授 (90217175)
Project Period (FY) 2002 – 2003
Project Status Completed(Fiscal Year 2003)
Budget Amount *help
¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 2003 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 2002 : ¥2,100,000 (Direct Cost : ¥2,100,000)
Keywordsp53 gene / DNA polymorphism / species identification / intron 7 / primates / evolution / p53遺伝子第7イントロン / アリル頻度 / 民族特異性 / 連鎖不平衡 / 人獣鑑別 / 分子系統樹
Research Abstract

1) The development of new species identification method using p53 gene analysis ::
Size and sequence of intron 7 of p53 gene which was included for PCR amplified product were made clear, after it sets the primer at the region (IV and V) which was preserved by the common amino acid of p53 gene between animal species.
In the primates, it was difficult to identify the species from the difference between the size of the PCR amplification product. In the other mammalian, it was possible specifically of the animal species, because it was different in size and base sequence of amplification product.
In the future, it seems to clarify it on molecule system evolution and junction of the animal by examining the p53 gene analysis of the other animal species including the mammalian.
2) Discrimination and evolution of the primates by the p53 gene analysis
The analysis of p53 gene exon 4, 5 and 6 of the human and 15 kinds of the non-human primates (monkeys) were carried out. When base sequences of human, chimpanzee and gorilla were compared, there was the base substitution in two places of the 81st (G→A) and 84th (C→T) of exon 5 in the chimpanzee, and in two places of 10th (G→A) and 114th (C→T) of exon 5 in the gorilla. It would be possible to distinguish human and anthropoid which was difficult until now.
When the homology of the base sequence was compared in the human and the monkey, it showed about 98.8% in the anthropoid, about 96.4% in the old world monkey, about 94.1% in the new world monkey and about 86.2% in the prosimians.
The lineage of 16 kinds of primates was analyzed by the preparation of the molecule genealogical tree by the mean distance method (UPGMA), it did not contradict a classification of conventional morphology.
It became clear that the analysis of the p53 gene base sequence of the primates was effective for elucidation of evolution and bifurcation of the human.

Report

(3results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

Research Products

(15results)

All 2004 2003 2002 Other

All Journal Article Publications

  • [Journal Article] 日本人集団におけるp53遺伝子のDNA多型解析-第7イントロンの多型性-2004

    • Author(s)
      安積順一, 魏慧玲, 藤井裕人, 松本博志, 玉木敬二
    • Journal Title

      DNA多型 12

      Pages : 111-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Analysis of DNA polymorphism of p53 gene in the Japanese population.2004

    • Author(s)
      Azumi, J., Gi E., Fujii H., Matsumoto H., Tamaki K.
    • Journal Title

      DNA Polymorphism 12

      Pages : 111-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] p53遺伝子解析による人獣鑑別に関する研究-ヒトと非ヒト霊長類の鑑別-2003

    • Author(s)
      安積順一, 松本博志, 濱田修平, 魏慧玲, 吉川摩由, 西田陽子, 玉木敬二, 竹中修
    • Journal Title

      DNA多型 11

      Pages : 19-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Studies on species identification using p53 gene analysis.2003

    • Author(s)
      Azumi, J., Matsumoto H., Hamada S., Gi E., Yoshikawa M., Nishitani Y., Tamaki K., Takenaka S.
    • Journal Title

      DNA Polymorphism 11

      Pages : 19-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] p53遺伝子を用いた人獣鑑別法の検討2002

    • Author(s)
      安積順一, 松本博志, 玉木敬二
    • Journal Title

      DNA多型 10

      Pages : 246-251

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Allelic structures at hypervariable minisatellite B6。7 in Japanese show populataion specificity。2002

    • Author(s)
      Mizukoshi T, Tamaki K, Azumi J, Matsumoto H, Imai K, Jaffreys AJ
    • Journal Title

      J Human Genet 47

      Pages : 232-238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Species identification using p53 gene.2002

    • Author(s)
      Azumi, J., Matsumoto H., Tamaki K.
    • Journal Title

      DNA Polymorphism 10

      Pages : 246-251

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Allelic structures at hypervariable minisatellite B6.7 in Japanese show population specificity.2002

    • Author(s)
      Mizukoshi T., Tamaki K., Azumi J., Matsumoto H., Imai K., Jeffreys AJ.
    • Journal Title

      J Human Genet 47

      Pages : 232-238

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 安積順一, 松本博志, 玉木敬二: "p53遺伝子を用いた人獣鑑別法の検討"DNA多型. 10. 246-251 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mizukoshi T, Tamaki K, Azumi J, Matsumoto H, Imai K, Jaffreys AJ: "Allelic structures at hypervariable minisatellite B6.7 in Japanese show populataion specificity"J Human Genet. 47. 232-238 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安積順一, 松本博志, 玉木敬二: "p53遺伝子解析による人獣鑑別に関する研究-ヒトと非ヒト霊長類の鑑別-"DNA多型. 11. 19-22 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安積順一, 魏慧玲, 藤井裕人, 松本博志, 玉木敬二: "日本人集団におけるp53遺伝子のDNA多型解析-第7イントロンの多型性-"DNA多型. 12(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安積順一, 松本博志, 玉木敬二: "p53遺伝子を用いた人獣鑑別法の検討"DNA多型. 10. 246-251 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizukoshi T, Tamaki K, Azumi J, Matsumoto H, Imai K, Jaifreys AJ: "Allelic structures at hypervariable minisatellite B6.7 in Japanese show populataion specificity"J Human Genet. 47. 232-238 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 安積順一, 松本博志, 玉木敬二: "p53遺伝子解析による人獣鑑別に関する研究-ヒトと非ヒト霊長類の鑑別-"DNA多型. 11(印刷中). (2003)

    • Related Report
      2002 Annual Research Report

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Published : 2002-04-01   Modified : 2016-04-21  

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