STUDIES ON HOST FACTORS RELATED TO FULMINANT HEPATITIS
| Project/Area Number |
14570444
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
YOKOSUKA Osamu CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, LECTURER, 大学院・医学研究院, 講師 (90182691)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUHISA Takeshi CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, PROFESSOR, 大学院・医学研究院, 教授 (20134364)
IMAZEKI Fumio CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, LECTURER, 大学院・医学研究院, 講師 (40223325)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | FULMINANT HEPATITIS / LIVER REGENERATION / PROTEIN SYNTHESIS / DNA MICROARRAY / OSTEOPONTIN / GENE EXPRESSION / OVAL CELL / IGFBP1 / DNAチップ / SNP解析 / サイトカイン / アポトーシス / FAS / FLIP |
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| Research Abstract |
Fulminant hepatitis is a poor prognostic liver disease characterized with massive death of hepatocytes and poor liver regeneration, however, host factors related to the fulminant hepatitis is not well known. First, we comprehensively analyzed the changes in gene expression during mouse liver regeneration using an in-house microarray. Genes involved in protein synthesis and posttranslational processing were enriched in the cluster characterized by rapid gene activation. Genes for plasma, protein secretion and intermediate metabolism were enriched in clusters exhibiting the features of gradual gene activation. Thus, clustering analysis of expression patterns of functionally classified genes gave insights into mechanism and pathophysiology of liver regeneration. Then, the gene expression profiles in oval cell induction models, mimicking fulminant hepatitis, were also examined with DNA microarray. Four genes including osteopontin were up-regulated in these models suggesting that these genes might be related to the development of fulminant hepatitis. The serum osteopontin level was revealed to be significantly higher in patients with fulminant hepatitis compared to those with acute self limited hepatitis. Further studies is uncle way to reveal the role of osteopontine during the development of fulmimant hepatitis.
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Report
(3 results)
Research Products
(9 results)
