Immunohistologial study on the expression of developmental intestinal epithelial antigens in embryogenesis and oncogenesis.
Project/Area Number |
14570499
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | Okayama Prefectural University |
Principal Investigator |
OKADA Yoshio Okayama Prefectural University, Faculty of Health and Welfare Science, Professor, 保健福祉学部, 教授 (30213953)
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Project Period (FY) |
2002 – 2004
|
Project Status |
Completed (Fiscal Year 2004)
|
Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
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Keywords | intestinal epithelia / apomucin / sucrase-isomaltase / Cdx / embryogenesis / development / differentiation / oncogenesis / 消化管 / 分化 / Cdx1 / Cdx2 / Musashi 1 / ランゲルハンス島 / 免疫組織染色 / ラット / 上皮細胞 / 発生分化 / 癌 / モノクローナル抗体 / ホメオティック遺伝子 / 大腸癌 / Sucrase-Isomaltase / CDX / モノクロナール抗体 |
Research Abstract |
1.Monoclonal antibodies that can be used for immunohistostaining of the formalin-fixed tissues were established to study the expression of the developmental antigens in rat embryonal and cancerous intestinal epithelia. Using the synthetic oligopeptides, mouse monoclonal antibodies against sucrase-isomaltase, MUC2 tandem and non-tandem repeat antigens, Cdx1, Cdx2, and Musashi1 were established. 2.Expression of developmental intestinal epithelial antigens was studied immunohistochemically using the newly established monoclonal antibodies and the fixed tissues of embryonal rat intestines. Cdx2 was found to be expressed in the nuclei of endodermal cells at day 11 pc, but Cdx1 was first detected at day 15 pc. As the intestinal villi developed, Cdx1 and Cdx2, respectively, tended to localize in the intervillous endoderms and the villous epithelia. Although both of MUC2 and sucrase-isomaltase were synthesized and expressed before birth, the former expression preceded three days of the latter expression that was first detected at day 19 pc. The expression of Cdxs was not simply correlated with those of MUC2 and sucrase-isomlatase. 3.Expression of apomucins, the blood group-related carbohydrate antigens and Cdx2 in the AOM-induced rat intestinal tumors was studied. Their expressions were generally suppressed in the tumors, though MUC5AC and H-antigen were observed to be expressed in the tissues not expressing them normally. Quantitative, but not qualitative, differences in the expression existed between the adenomas and the cancers. Although B-antigen expressed in the proximal colon might be carried by MUC5AC, there was no apparent association between the expressions of apomucins, the carbohydrate antigens and Cdx2. Expression of Cdx2 was almost completely suppressed in the AOM tumors.
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Report
(4 results)
Research Products
(9 results)