New gene therapy for lung cancer by suppressor of cytokine signaling-1
| Project/Area Number |
14570552
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Ehime University |
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Principal Investigator |
HAMADA Hironobu Ehime University, School of Medicine, instructor, 医学部附属病院, 助手 (80314954)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIMA Miki Ehime University, School of Medicine, instructor, 医学部, 助手 (90363225)
YOKOYAMA Akihito Hiroshima University, Graduate School of Biomedical Sciences, lecturer, 医学部, 講師 (30191513)
NAKA Tetsuji Osaka University, Graduate School of Medicine, instructor, 医学部, 助手 (30303936)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Lung cancer / STAT3 / SOCS-1 / Malignant mesothelioma |
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| Research Abstract |
Background : Aberrant activation of signal transducer and activator of transcription (STAT) 3 via abnormal signaling of the Janus kinase (JAK)/STAT pathway has been demonstrated to directly contribute to oncogenesis in hematologic and non-hematologic malignancies. Loss of SOCS-1, a negative regulator of JAK/STAT pathway, has been reported to lead to STAT3 activation and cell proliferation.
Objective : We examined role of SOCS-1 in the pathogenesis of malignant mesothelioma.
Methods : We used five malignant mesothelioma cell lines. Western blot analysis was performed to detect tyrosine phosphorylation status of STAT3. Northern blot analysis was used to detect expression level of SOCS-1 mRNA. SOCS-1 gene was transfected to the cell lines using adenovirus vector.
Results : Both tyrosine phosphorylated STAT3 and decreased expression of SOCS-1 mRNA were observed in three of five mesothelioma cell lines. The effect of SOCS-1 restoration was tested in these three cell lines and significant decrease of growth rate was observed in two of three cell lines.
Conclusion : These results suggested that decreased levels of SOCS-1 expression may lead to aberrant activation of STAT3 and that the restoration of SOCS-1 may be a new therapeutic strategy for the treatment of malignant mesothelioma.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Expression patterns of sialylated epitope recognized by KL-6 monoclonal antibody in ocular surface epithelium of normals and dry eye patients.2004
Author(s)
Hayashi Y, Kao WW, Kohno N, Nishihara-Hayashi M, Shiraishi A, Uno T, Yamaguchi M, Okamoto S, Maeda M, Ikeda T, Hamada H, Kondo K, Ohashi Y.
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Journal Title
Investigative Ophthalmology Visual Science 45
Pages: 2212-2217
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Circulating KL-6 levels in patients with drug induced pneumonitis.2003
Author(s)
Ohnishi H, Yokoyama A, Yasuhara Y, Watanabe A, Naka T, Hamada H, Abe M, Nishimura K, Higaki J, Ikezoe J, Kohno N.
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Journal Title
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Comparative study of KL-6,SP-A,SP-D, and MCP-1 as serum markers for interstitial lung diseases.2002
Author(s)
Ohnishi H, Yokoyama A, Kondo K, Hamada H, Abe M, Nishimura K, Hiwada K, Kohno N.
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Journal Title
American Journal of Respiratory and Critical Care Medicine 165
Pages: 378-381
Description
「研究成果報告書概要(欧文)」より
Related Report
