Research for new tumor suppressor gene and mechanism of oncogenisity of Wilms tumors
| Project/Area Number |
14570770
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kitasato University |
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Principal Investigator |
NAKADATE Hisaya Kitasato Univ., School of Medicine, Assistant Professor, 医学部, 講師 (30306633)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Yasuhiko SAITAMA CANCER CENTERHOSIPTAL, DEPARTMENT OF DAY CARE, CHIEF PHYSICIAN, デイケア部, 部長(研究職) (50373387)
松浦 信夫 北里大学, 医学部, 教授 (50002332)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | WILMS TUMOR / WT1 / TSLC1 / LOH / METHYLATION |
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| Research Abstract |
Allelic loss on 11p and 11q
Of the sporadic 98 tumors in which allelic loss on 11p and 11q was examined had at least 1 informative locus on 11p. Of the 34 sporadic and unilateral tumors, 11p LOH was 23.5% of cases, LOH on 11q was 9.4%, 16.3% had 11q23 LOH. LOH limited to the 11p13 region was 16.3%, 11p15 LOH was 17.3%, LOH in the whole 11p15 - 11p13 region was 3.1%.
Therefore common LOH region were limited to 11p15, 11p13, 11q23. TSLC1, lung cancer tumor suppressor gene is on 11q23 region. So, we investgated TSLC1 as a candidate tumor suppressor gene of the Wilms tumors.
Deletion/mutation and methylation analysis of TSLC1
With 34 LOH cases, there were no deletion/mutation cases. By bisulfate method, methylation analysis of TSLC1 was studied. 11q23 LOH cases, 21.4%, partial methylated, none were completed methylated. We concluded that TSLC1 was not a new candidate suppressor gene of the Wilms tumors, but this gene may have some effects on oncogenesis of the Wilms tumors.
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Report
(4 results)
Research Products
(16 results)
