• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of the methods to detect enzyme activities of proteins involved in repair of DNA double strand breaks

Research Project

Project/Area Number 14570871
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionSAPPORO MEDICAL UNIVERSITY

Principal Investigator

SAKATA Koh-ichi  Sapporo Medical University, School of Medicine, Associate professor, 医学部, 助教授 (10235153)

Co-Investigator(Kenkyū-buntansha) HAREYAMA Masato  Sapporo Medical University, School of Medicine, professor, 医学部, 教授 (10173098)
OOUCHI Atsushi  Sapporo Medical University, School of Medicine, Instructor, 医学部, 助手 (70168863)
NAGAKURA Hisayasu  Sapporo Medical University, School of Medicine, Instructor, 医学部, 助手 (80244359)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsDNA-dependent protein kinase / genomic instability / chromosomal aberration / cancer risk / peripheral blood lymphocytes
Research Abstract

The presence of genomic instability in cells is known to play an important role in the multistage carcinogenesis of various organs. The DNA double strand breaks(DSBs) repair pathway has been implicated in maintaining genomic integrity via suppression of chromosomal rearrangements. DNA-dependent protein kinase (DNA-PK) has an important role in DNA DSBs repair. The purpose of this study was to determine how DNA-PK activity varies among untreated cancer patients and cancer-free normal healthy volunteers and how this affects cancer risk. DNA-PK activities of peripheral blood lymphocytes(PBL) in normal volunteers were 15.84±4.87 pmol and those of cancer patients were 12.28±5.10 pmol. There was the significant difference between them (p=0.012). Age and smoking had no association with DNA-PK activity. No association was found between DNA-PK activity and expression of DNA-PKcs, ku70, and ku86. A relationship between DNA-PK acitvity and chromosome aberration in PBL was shown to exist. Dicentric chromosomes were not observed in individuals whose DNA-PK activity was higher than 16.32 pmol. The frequency of excess fragment increased as the DNA-PK activity decreased. We conclude that DNA-PK activity is associated with chromosomal instability. DNA-PK activity in PBL is associated with risk of various kinds of cancer. Therefore, DNA-PK activity in PBL would be useful as a tumor marker to assist in the diagnosis of cancer and in the prediction of cancer susceptibility.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

URL: 

Published: 2002-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi