| Project/Area Number |
14571020
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Faculty of Medical Sciences, University of Fukui |
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Principal Investigator |
KONOSHITA Tadashi University of Fukui, Third Department of Internal Medicine, Faculty of Medical Sciences, Associate Professor, 医学部, 助教授 (40270954)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMORI Isamu University of Fukui, Third Department of Internal Medicine, Faculty of Medical Sciences, Professor, 医学部, 教授 (40142278)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | renin / prorenin / convertase |
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| Research Abstract |
For long time, it has been unclear about genuine convertase for prorenin to renin, active form of the enzyme. Until now, glandular kallikrein (PRECE), athepsin B, PC1/3 and PC5/6 were regarded as the candidate enzymes. However, the specificity and activity were not enough for the genuine enzyme. This project aimed the cloning and characterization of the genuine prorenin convertase with renal RNA substraction and degenerate primer PCR for Kex family method. Gained clones were known ones. So that, as conclusion, so far, it is thought that there exist no prorenin convertase in Kex family in kidney.
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