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The role of co-factor of estrogen receptor alfa in breast cancer cells

Research Project

Project/Area Number14571149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionAichi Cancer Center Research Institute(2003)
Nagoya City University(2002)

Principal Investigator

TOYAMA Tatsuya  Aichi Cancer Center Research Institute, researcher, 研究所, 研究員 (30315882)

Co-Investigator(Kenkyū-buntansha) IWASE Hirotaka  Nagoya City University Medical School, Associate Professor, 大学院・医学研究科, 助教授 (40211065)
山下 啓子  名古屋市立大学, 大学院・医学研究科, 助手 (70332947)
Project Period (FY) 2002 – 2003
Project Status Completed(Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 2003 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Fiscal Year 2002 : ¥2,400,000 (Direct Cost : ¥2,400,000)
Keywordsbeast cancer / estrogen receptor alfa
Research Abstract

The ING1 gene was originally cloned as a candidate tumor suppressor of human breast cancer, and recent studies suggest that ING1 proteins are involved in chromatin remodeling functions via physical association with both histone acetyltransferases and histone deacetylases. In this study, we investigated whether p33^<ING1b>, one of the major ING1 isoforms, modulated the transcriptional activity of estrogen receptor (ER) a. In Cos-7 cells transfected with increasing in a dose-dependent manner. As p33^<ING1b> expression levels increased, transcription of an ER-responsive reporter gene by either estrogen-inducible full-length ERa or activation function (AF) 1 deletion mutant was enhanced, while the AF2 deletion mutant was unaffected by the presence of p33^<ING1b>. These results showed that p33^<ING1b> enhanced estrogen-induced ERa activity through the AF2 domain. Our data also demonstrated that the antiestrogens inhibited the transcriptional activity of ERa as stimulated by p33^<ING1b>. Furthermore, a weak physical association was observed between in vitro translated p33^<ING1b> and ERa. Our data presented here demonstrate that p33^<ING1b> acts like a coactivator for ERa and stimulates estrogen-induced ERa transcriptional activity consistent with a function for p33^<ING1b> in chromatin remodeling.

Report

(3results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report

Research Products

(3results)

All Other

All Publications

  • [Publications] Toyama T, et al.: "p33ING1b stimulates the transcriptional activity of the estrogen receptor alfa via its activation function (AF) 2 domain"Journal of Steroid Biochemistry and Molecular Biology. 87・1. 57-63 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyama T, et al.: "p33ING1b stimulates the transcriptional activity of the estrogen receptor alfa via its activation function (AF) 2 domain"J Steroid Biochem Mol Biol. 87(1). 57-63 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyama T, et al.: "p33ING1b stimulates the transcriptional activity of the estrogen receptor a via its activation function (AF) 2 domain"Journal of Steroid Biochemistry and Molecular Biology. 87・1. 57-63 (2003)

    • Related Report
      2003 Annual Research Report

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Published : 2002-04-01   Modified : 2016-04-21  

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