Bioartificial pancreas transplantation for Type 1 diabetes
| Project/Area Number |
14571153
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Nara Medical University |
|---|
Principal Investigator |
HISANAGA Michiyoshi Nara Medical Univ., Faculty of Medicine, associate professor, 医学部, 助教授 (30275341)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAGAO Mitsuo Nara Medical Univ., Faculty of Medicine, assistant professor, 医学部, 助手 (10326340)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | Type-1 diabetes / Pancreatic islet transplantation / Agarose microcapsulation / Bioartificial Pancreas / Autoimmune reaction |
|---|
| Research Abstract |
[Aim] The recurrence of autoimmunity and allograft rejection act as major barriers to the widespread use of islet transplantation as a cure for type 1 diabetes. The aim of this study was to evaluate the feasibility of immunoisolation by use of an agarose microcapsule to prevent autoimmune recurrence after islet transplantation.
[Methods] Highly purified islets were isolated from 6 to 8-week-old prediabetic male nonobese diabetic (NOD) mice and microencapsulated in 5% agarose hydrogel as a semipermiable membrane. Islet function was evaluated by a syngeneic islet transplantation model, in which islets were transplanted into spontaneously diabetic NOD mice.
[Results] The nonencapsulated islet grafts were destroyed and diabetes recurred within 2 weeks after transplantation in all 12 mice. In contrast, 13 of 16 mice that underwent transplantation with microencapsulated islets maintained normoglycemia for more than 100 days after islet transplantation. Histologic examination of the nonencapsulated islet grafts showed massive mononuclear cellular infiltration with β-cell destruction. In contrast, the microencapsulated islets showed well-granulated β-cells with no mononuclear cellular infiltration around the microcapsules or in the accompanying blood capillaries between the microcapsules.
[Condusions] Agarose microcapsules were able to completely protect NOD islet isografts from autoimmune destruction in the syngeneic islet transplantation model.
|
Report
(3 results)
Research Products
(2 results)
