Mechanism of latent infection of Herpesviridae and its reactivation following organ transplantation
| Project/Area Number |
14571159
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Tokai University |
|---|
Principal Investigator |
TANAKA Kazuo Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (50236569)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAWAMURA Sadaki Tokai University, School of Medicine, Assistant Researcher, 医学部, 助手 (80056241)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | cytomegalovirus / real-time PCR / infectious immunity / memory cells / MHC / tetramer / germfree mouse / LPS / CpG motif / サイトムガロウイルス / メモリーT細胞 / 常在細菌叢 / リポ多糖 / 抗原4量体 |
|---|
| Research Abstract |
BALB/c mice were ip infected with murine cytomegalovirus (MCMV). When virus titers were enumerated by real-time PCR, it was demonstrated that the virus was latently infected in the lungs 3 months after the infection. However, a large number (Ca.10% of total pulmonary lymphocytes) of MCMV-specific memory-effctor CTL, which was examined using MHC/antigen-tetramer, was still detectable in the organs even 1 year after the infection. Thus, MCMV-specific memory-effctor CTL was presumably maintained without MCMV antigens. Thus, MCMV-specific memory-effctor CTL may play a role in prevention of CMV-reactivation.
On the other hand, such MCMV-specific memory-effctor CTL could not be detected in the lungs of germfree mice, suggesting that such bacterial product as LPS and/or CpG motif may participate in the maintenace of MCMV-specific memory-effctor CTL. This result suggested that eradication of intestinal bacterial flora in pre-treatment before bone marrow transplantation may diminish indigenous MCMV-specific memory-effctor CTL.
|
Report
(3 results)
Research Products
(17 results)
