Basic research for molecular targeting therapy against gastro-intestine cancer

Research Project

Project/Area Number	14571163
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General surgery
Research Institution	Fujita Health University
Principal Investigator	AKAHORI Yasushi Fujita Health University, Institute for Comprehensive Medical Science, Assistant, 総合医科学研究所, 助手 (80221711)
Project Period (FY)	2002 – 2003
Project Status	Completed (Fiscal Year 2003)
Budget Amount *help	¥3,000,000 (Direct Cost: ¥3,000,000) Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000) Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywords	antibody / KDR / CEA / HER2 / TRAIL-R2 / gastro-intestine / cancer / ヒト抗体ライブラリー / 癌抗原 / 細胞表面タンパク / 有機溶媒層 / スクリーニング
Research Abstract	(1)Antibody isolation against KDR KDR domain2 was expressed as His-tag form in E.coli pET system and used as antigen for antibody screening. After three round of screening of antibody library, we isolated ten antibody clones that recognized the antigen. Two of them stained endotherial cells of gastric cancer and three clones stained tumor cells in colon cancer. Those observation shows that these antibodies recognize tumor tissue. (2)Antibody isolation against CEA, HER2, TRAIL-R2 We further isolated anti-CEA, HER2, TRAIL-R2 antibodies by the screening of antibody library. These antibodies recognized tumor cell lines. Conversion of these antibodies to full human IgG form did not changed their characteristics. One anti-HER2 IgG form antibody showed ADCC activity.