| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Yasuyuki Gifu University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (90211309)
NAGAO Narutoshi Gifu University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (80334944)
佐治 重豊 岐阜大学, 医学部, 教授 (80021400)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
Aurora family are highly expressed during M phase, and have been suggested to regulate centrosome function, chromosome segregation, and cytokinesis. In addition, PLK family is also intimately involved in spindle formation and chromosome segregation during mitosis. The purpose of this study was to determine whether PLK or Aurora family are overexpressed in primary colorectal cancer specimens as compared with normal colon mucosa and assess its relation to other kinases as a potential new tumor marker.
1.PLK
In the present study, immunohistochemical analyses were performed of PLK1 expression in 78 primary colorectal cancers as well as 15 normal colorectal specimens. Furthermore, we examined the relationship between other kinases, Aurora-A and Aurora-C, and PLK1 expression. In normal colon mucosa, some crypt cells showed weak positive staining for PLK1 in 13 out of 15 cases. Elevated expression of PLK1 was observed in 57 (73.1%) of the colorectal cancers, statistically significant associatio
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ns being evident with pT, pN and the Dukes' classification. Values for lesions with high and low PLK1 expression were 54.7±10.3% (mean±SD) and 45.9±11.9%. PLK1 was significantly associated with Aurora-A, but PLK1 staining was more diffuse and extensive than for Aurora-A or Aurora-C.
2.Aurora
In the present study, immunohistochemical analyses were performed of HsAIRK1 and HsAIRK3 expression in 78 primary colorectal cancers and 36 colorectal adenomas as well as 15 normal colorectal specimens. Elevated expression of HsAIRK1 was observed in 53 (67.9%) of the colorectal cancers, and of HsAIRK3 in 40 (51.3%). Furthermore, colorectal adenomas showed high expression of HsAIRK1 and HsAIRK3 in 11 (30.6%) and 7 (19.4%) cases, respectively, thus being intermediate between colorectal cancers and normal colorectal mucosa. Interestingly, HsAIRK1 overexpression was significantly associated with p53 accumulation in colorectal cancers.
Our results suggest overexpression of PLK1 or Aurora A might be of pathogenic, prognostic and proliferative importance, so that these mitosis-related genes might have potential as a new tumor marker for colorectal cancers. Less
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