| Project/Area Number |
14571209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIMIZU Shuji Fac.Med., associate professor, 大学病院, 助教授 (70274454)
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| Co-Investigator(Kenkyū-buntansha) |
CHIJIIWA Kazuo Miyazaki Univ.School Med., professor, 医学部, 教授 (90179945)
YAMAGUCHI Koji Kyushu University, Graduate School of Medical Sciences, associate professor, 大学院・医学研究院, 助教授 (50191226)
KUROKI Syoji Fac.Med., lecturer, 大学病院, 講師 (30215090)
YAMAGUCHI Tokio Tokyo Dent.Med.Univ., associate professor, 難知疾患研究所, 助教授 (30134745)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | bilirubin / superoxide / hyperbaric oxygen / jaundice / biopyrrin / lipid peroxides / acrolein / 8-hydroxy deoxyguanosine / 黄疸 / 高圧酸素療法 / 酸化ストレス / 尿 / 抗酸化剤 |
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| Research Abstract |
【Aim】 The aim of the present study is to examine the effect of hyperbaric oxygen on the hyperbiirubinemia patients from the standpoint of antioxidant action of bilirubin. 【Methods and patients】 The serum and urine were collected from 15 jaundiced patients before and after hyperbaric oxygen with 6 non-jaundiced patients as control. 【Results】 In the jaundiced patients, lipid peroxides (MDA) significantly increased from 3.0±1.0 nmol/ml to 3.3±1.1 nmol/ml (p=0.02). In the non-jaundiced patients, 8-hydroxy deoxyguanosine increased from 15.2±7.4 μg/g creatinine to 15.7±7.5 μg/g creatinine (p=0.08). The level of bilirubin oxidative mnetabolites (BOM) (p<0.01), MDA (p=0.07) and acrolein (p=0.08) were higher in the jaundiced than the non-jaundiced patients before hyperbaric treatment. There were significant correlations between bilirubin and BOM (y=115x+1.8, r=0.845, p<0.0001), bilirubin and MDA (y=0.189x-52, r=0.794, p <0.0001), and BQM and MDA (y=0.001x+2.1, r=0.746, p<0.0001).【Conclusions】 Superoxides were produced by hyperbaric oxygen therapy both in the jaundiced and the non-jaundiced patients. Jaundiced patients were in hyperoxidative state even before the treatment, where bilirubin acted as a scavenger of the superoxides.
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