Clinical and experimental research on prolongation of durability of cryopreserved allogeneic heart valve and vessel
| Project/Area Number |
14571258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ONO Minoru The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (40270871)
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| Co-Investigator(Kenkyū-buntansha) |
MOTOMURA Noboru The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (40332580)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Allograft / heart valve / vascular vessel / Cryopreservation / Aortic implantation |
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| Research Abstract |
The descending thoracic aorta of Lewis rat was implanted into infrarenal abdominal aorta as fresh graft or after cryopreservation more than a week. Cryopreservation was conducted in the same way as used for human tissues. Syngeneic and allogeneic (to BN rat) implantation was performed. The grafts were explanted one and four week(s) after implantation. They were stained by HE and immunohistochemical methods for CD4, CD8a and CD83.
Mixed lymphocyte reaction was performed to compare antigenicity between allogeneic and syngeneic implantation. Spleen lymphocytes of recipient were used as responder cells. Lymphocytes of naive BN rat were served as control. Lymphocytes were harvested one and four week(s) after implantation to examine time-related intensity change. Stimulator cells were harvested and pretreated by Mitomycin C (Lewis, BN, and DA rats as a third party).
Graft patency was 100% in syngeneic group, 83.3% in allo-fresh group and 66.7% in allo-cryo group. HE staining showed marked infiltration of lymphocytes and mononuclear cells, particularly on the adventitial side in both allo-fresh and allo-cryo groups. Intimal hyperplasia and lymphocyte infiltration on the intima were also seen in some rats. There was little inflammatory cell infiltrate either on the adventitial or on the intimal side in syngeneic group. A number of CD83-positive cells were found among infiltrating cells in allogeneic group. CD4-positive and CD8a-positive cells were also found. Infiltrating cells were much larger in number in allogeneic group, compared to syngeneic group in cryopreserved graft implantation as well as fresh setting. Intensity of cellular infiltration was stronger in fresh graft than cryopreserved one in allogeneic group. MLR showed significantly higher reaction in both allogeneic group than in syngeneic group. There was no intensity difference between fresh and cryopreserved grafts. No time-related change was detected in the intensity after one week post-implantation.
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Report
(4 results)
Research Products
(27 results)
