Establishment of a novel therapy for glioma invasion by antisense inhibition of the targeting molecule.
| Project/Area Number |
14571314
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Ehime University |
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Principal Investigator |
OHNISHI Takanori Ehime University, School of Medicine, professor, 医学部, 教授 (70233210)
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Hironobu Ehime University, University Hospital, Assistant, 医学部附属病院, 助手 (20335897)
NAKAGAWA Kou Ehime University, University Hospital, Assistant professor, 医学部附属病院, 講師 (50155678)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | GLIOMA / TUMOR INVASION / LAMININ-8 / ANTISENSE |
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| Research Abstract |
In order to clarify the molecular mechanism of glioma invasion, we studied an expression of laminin-8 in brain tumors and investigated an effect of antisense inhibition of laminin-8 on migration and invasion of glioma cells. The protein and mRNA of laminin-8 were highly expressed in malignant gliomas, particulary in glioblastomas showing diffuse invasion. Antisense of laminin-8(α4 subunit) markedly inhibited the glioma cell migration and invasion in vitro without affecting the cell growth. In addtion, antisense of laminin-8 significantly inhibited the glioma cell invasion in both a rat brain slice model and a tumor-inoculated model. Antisense inhibition of laminin-8 resulted in weak formation of actin stress fibers in glioma cells but did not affect the expression of Rac and Cdc42. These results suggest that gene suppression of laminin-8 may inhibit the glioma cell motility through the inhibition of formation of focal adhesion.
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Report
(3 results)
Research Products
(16 results)
