Bone and cartilage regeneration by peripheral blood derived stem cell
| Project/Area Number |
14571381
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TANAKA Hiroshi Yamaguchi University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (50197464)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | peripheral blood derived cell / bone marrow stromal cell / ES cell / osteoblast / chondrocyte / bone formation / cartilage regeneration / gene expression / 抹消血幹細胞 / 成長因子 / 末梢血管細胞 |
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| Research Abstract |
Cultured cells from peripheral blood or bone marrow were treated with dexamethasone or growth factors. Cells were determined for alkaline phosphatase activity and analyzed by RT PCR for osteoblast markers. Alkaline phosphatase-positive cells were present in bone marrow cells, but not in peripheral blood-derived cells. Gene expression for osteoblast markers were increased in bone marrow cells
Differentiation for chondrocyte
Peripheral blood-derived cells, bone marrow cells, fibroblasts, or ES cells were cultured in three-dimensional structure, such as pellet or micromass. Dexamethasone or growth factors were added for three weeks. Histological and immunohistochemical analysis of pellet cultures of bone marrow cells, fibroblasts, and ES cells, but not peripheral blood-derived cells, revealed cartilage-like tissue characterized by metachromatically stained extracellular matrix and type II collagen immunoreactivity, indicative of chondrogenesis
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Report
(3 results)
Research Products
(4 results)
