Mechanism of calcification on regerative articular cartilage

• Project/Area Number: 14571395
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Teikyo University (2003); Kyoto Prefectural University of Medicine (2002)
• Principal Investigator: TAKEI Shinro Teikyo University, Medicine, Professor, 医学部, 教授 (10226730)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥4,000,000 (Direct Cost: ¥4,000,000); Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
• Keywords: articular cartilage / osteochondral defect / tissue regeneration / tidemark / mesenchymal cell / autologous cell transplantation / transgenic rat / in situ hybridization / 軟骨石灰化前線

Research Abstract

The research which used the autologous cell graft for joint cartilage defect restoration has accomplished mostly. Restoration of normal hyaline cartilage has not been attained. We have completed the method for distinguishing donor and recipient cells using the combination of transgenic rat and DNA in situ hybridization. We reported those in the 48th and 49th Annual Meeting of the Orthopaedic Research Society (ORSI US), and the 18th Japanese Orthopaedic Association Basic Scientific Meeting, 2002, and 2003 USA, 2002, 2003. Using this technique, it was clearly demonstrated that cells from surrounding bone marrow replace the underlining bone of the donor with the donor cells. The model of following the transplanted cells was completed using GFP transgenic rats. This method is more reliable than the previously reported method using transgenic rat and DNA in situ hybridization. The results were reported in the 50th Annual Meeting of the Orthopaedic Research Society (ORS, US).

Research Products

• [Publications] Oshima, Y., Watanabe, N., Matsuda, K., Takenaka, N., Kawata, M., Takai, S.: "Behavior of Graft and Host Cells in Underlying Subchondral Bone After Transplantation of Osteochondral Autograft."Microscopy Research and Technique. 58・1. 19-24 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Watanabe, N., Takai, S., Kawata, M., Hirasawa, Y.: "Introduction for"Donor transplant vs. recipient cells in musculoskeletal System""Microscopy Research and Technique. 58・1. 1-2 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Watanabe, N, Woo, SLY., Papageorgion C., Celechovsky C., Takai, S.: "Fate of Donor Bone Marrow Cells in Medial Collateral Ligament after Simulated Autologous Transplantation."Microscopy Research and Technique. 58・1. 39-44 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 高井信朗, 渡邉信佳, 大島康史, 竹中信之: "自家骨軟骨移植術後の軟骨層および軟骨下骨層のリモデリング"炎症と免疫. 11・5. 576-582 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Oshima, Y., Takai, S., Watanabe, N., Matsuda, K., Kawata, M., Kubo, T.: "Fate of Autologously Transplanted Bone Marrow-Derived Mesenchymal Cells during repair process of Full-Thickness Articular Cartilage Defect"(投稿中).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Oshima, Y., Watanabe, N., Matsuda, K., Takenaka, Kawata, M., Takai, S.: "Behavior of Graft and Host Cells in Underlying Subohondral Bone After Transplantation of Osteochondral Autograft"58(1). 19-24 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Watanabe, N., Takai, S., Kawata, M., Hirasawa, Y.: "Introduction for "Donor transplant vs. recipient cells in musculoskeletal system""58(1). 1-2 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Watanabe, N., Savio L-Y.Woo, Christos Papageorgiou, Chris Celechovsky, Takai, S.: "Fate of Donor Bone Marrow Cells in Medial Collateral Ligament after Simulated Autologous"Microscopic Research Technique. 58(1). 39-44 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oshima, Y., Takai, S., Watanabe, N., Matsuda, K., Kawata, M., Kubo, T.: "Fate of Autologously Transplanted Bone Marrow-Derived Mesenchymal Cells during repair process of Full-Thickness"(Submitted to the Journal).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 高井信朗, 渡邊信佳, 大島康史, 竹中信之: "自家骨軟骨移植術後の軟骨層および軟骨下骨層のリモデリング"炎症と免疫. 11巻5号. 576-582 (2003)
• [Publications] Ohima Y, Watanabe N, Matsuda K, Takenaka N, Kawata M, Takai S: "Behavior of Graft and Host Cells in Underlying Subchondral Bone After Transplantation of Osteochondral Autograft"Microscopy Research and Technique. 58(1). 19-24 (2002)
• [Publications] Watanabe N, Woo SL, Papageorgiou C, Celechovsky C, Takai S: "Fate of donor bone marrow cells in medial collateral ligament after simulated autologous transplantation"Microscopy Research and Technique. 58(1). 39-44 (2002)
• [Publications] Oshima Y, Watanabe N, Matsuda K, Takenaka N, Kawata M, Takai S: "Behavior of Graft and Host Cells in Underlying Subchondral Bone after Transplantation of Osteochondral Autograft"Transaction of International Cartilage Repair Society. 4. 3605 (2002)
• [Publications] 大島康史, 高井信朗, 渡邉信佳, 松田賢一, 河田光博, 久保俊一: "骨髄間葉系細胞塊移植による骨軟骨欠損の修復過程-Hanging Drop Culture法を用いて-"日本整形外科学会雑誌. 76(8). 51093 (2002)
• [Publications] Oshima Y, Takai S, Watanabe N, Matsuda K, Kawata M, Kubo T: "Behavior of transplanted bone marrow-derived mesenchymal cell-masses during the regenerating process of osteochondral defect using transgenic rats"Transaction of Orthopaedic Research Society. 28. 357 (2003)