Identification of factors involved in insulin resistance during pregnancy

• Project/Area Number: 14571554
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Mie University
• Principal Investigator: SUGIYAMA Takashi Mie University, School of Medicine, Associate Professor, 医学部附属病院, 助教授 (10263005)
• Co-Investigator(Kenkyū-buntansha): KUSAKA Hideo Mie University, School of Medicine, Assistant Professor, 医学部附属病院, 助手 (70332715) ; 豊田 長康 三重大学, 医学部, 教授 (40126983)
• Project Period (FY): 2002 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥2,800,000 (Direct Cost: ¥2,800,000); Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: insulin resistance / pregnancy / adiponectin / adipocyte / アデイポサイトカイン / マウス / 脂肪細胞 / adipocytokine / adiponectin / resistin

Research Abstract

The fat-derived factor adiponectin is downregulated in insulin resistance and obesity and insulin resistance has been demonstrated during late pregnancy in both humans and in rodents. The present study examines the physiological change of adiponectin gene expression, as well as the circulating levels of adiponectin during pregnancy. We examined the relative quantity of adiponectin mRNA produced in the adipose tissues of groups of mice during pregnancy and virgin mice. Serum levels of adiponectin and the size of parametrial adipocytes were also measured in mice throughout pregnancy. Adiponectin mRNA was significantly reduced by 74±8% and 63±4% at days 15 and 18 of pregnancy compared with virgin mice. The serum adiponectin concentration decreased on days 15 (30.7±8.5μg/ml) and 18 (27.9±8.7μg/ml) of pregnancy and the values were significantly lower than that of virgin mice (56.8±6.6μg/ml). Parametrial adipocytes from mice on days 15, 18 of pregnancy were significantly larger than those of virgin mice or during early pregnancy. Fat cell size and the degradation of adiponectin gene expression and serum adiponectin levels were closely correlated. These results suggest that changes of adiponectin expression affect metabolic status in pregnant mice

Research Products

• [Journal Article] Adiponectin mRNA levels in parametrial adipose tissue and serum adiponectin levels are reduced in mice during late pregnancy (2004)
  • Author(s): Kondo E, Sugiyama T, Kusaka H, Toyoda N
  • Journal Title: Hormone and Metabolic Research 36 Pages: 465-469
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kondo E, Sugiyama T, Kusaka H, Toyoda N: "Adiponection mRNA levels in parametrail adipose tissue and serum adiponectin levels are reduced in mice during late pregnancy"Hormone and Metabolic Research. (in press). (2004)