| Project/Area Number |
14571556
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
NODA Yoichi Shiga Univ, of Medical Science, OBGYN, Professor, 医学部, 教授 (50115911)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Sakae OBGYN, Instructor, 医学部, 助手 (70293826)
TAKEBAYASHI Koichi OBGYN, assistant professor, 医学部, 講師 (20303779)
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| Project Period (FY) |
2002 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | enbryo-trophicfactor / 2step embryo transfer / In Vitro Fertilization-Embryo Transfer |
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| Research Abstract |
As a result of our interdisciplinary research for the effectiveness of 2-step (consecutive) embryo transfer (ET) method, we concluded that 2-step ET is an effective option even in the case of poor responder or repeated failure. This procedure can avoid cancellation of ET and may benefit patients who can obtain a sufficient number of embryos. According to the analysis of LC-Mass spectrometry, we identified one of the embryo-derived factor, follistatin related gene (FLRG) protein, which is also expressed in human endometrium. In the proliferative phase of the menstrual cycle, FLRG protein was detected predominantly in the cytoplasm of endometrial epithelium. In the secretory phase and in early pregnancy, it was also detected in the nuclei of endometrial stromal cells (ESC). Using in vitro decidualization model, we demonstrated that 17β-estradiol (E2) plus progesterone (P), but not E2 or P alone, induced FLRG expression significantly. These results suggest that FLRG expression in ESC is regulated by the concerted action of ovarian steroid hormones via decidualization and FLRG protein may participate in the regulation of stromal cell decidualization as a binding protein for the members of TGF-β superfamily.
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