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The quantitative analyses of electroretinogram and histology for retinal degeneration using transgenic mice

Research Project

Project/Area Number 14571676
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKyushu University

Principal Investigator

GOTO Yoshinobu  Kyushu University, Faculty of Medicine, Assistant Professor, 大学院・医学研究院, 講師 (30336028)

Co-Investigator(Kenkyū-buntansha) TOBIMATSU Shozo  Kyushu University, Faculty of Medicine, Professor, 大学院・医学研究院, 教授 (40164008)
SAKAMOTO Taiji  Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (10235179)
Project Period (FY) 2002 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsretinal degeneration / transgenic models / electroretinogram / rods / cones / function of inner layer of retina / dark reared / gene therapy / 網膜色素変成症 / CMYCマウス / 杆体細胞 / 細胞変性 / 網膜内層 / 網膜色素変性症 / 杵体細胞 / 錐体細胞 / ON型,OFF型双極細胞 / 律動様小波
Research Abstract

The electrophysiological dysfunction of retina in retinitis pigmentosa should be caused by the retinal cells degeneration. The purposes of this project were to clarify the mechanism of this dysfunction and to advocate the new treatment in retinitis pigmentosa using the transgenic mouse and rat models. To address these issue, we quantitatively analyzed the electroretinograms and the histopathological changes of retina to evaluate the functional changes of rods, cones and inner cell layer in each transgenic mouse. We also compared the retinal functions between cyclic-light-reared and dark-reared mice to evaluate the retinal damages by the light. In addition, we transplanted the growth factor gene in subretina using the viral vector, and analyzed the protecting effects from the retinal degeneration. Our results were summarized as follows ; 1)the functions of ON- and OFF- bipolar cells in CMYC mouse were kept until 1 month, but disappeared at 6 month, 2)the dysfunctions of inner layer of r … More etina in each transgenic animal were started at earlier than the histological changes, 3)the dark-reared for 3 months was slowed the retinal degeneration in CMYC mouse, 4)the protecting effects for retinal functions by gene transplantation was observed in res rats (Ikeda et al.,2003;Miyazaki et al.,2003), and 5) there was not statistically significant the protecting effects for retinal functions by gene transplantation in dark-reared CMYC mouse. Overall, our findings suggest that the functional changes of rods, cones, and inner layers of retina were caused by the retinal cell degenerations. According to the new treatment, at present, the decrement of the light should be the first choice to protect the remaining functions of retina in retinal degeneration disease. In addition, the gene therapy should become a good treatment for this disease in near future, however we must improve the more practical methods of the vector transplantation to the retina. Finally, we could get the beneficial results at this project for the mechanism of functional decrements of retina and the treatment for the retinal degeneration in retinitis pigmentosa. Less

Report

(4 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • Research Products

    (17 results)

All 2004 2003 2002 Other

All Journal Article (10 results) Publications (7 results)

  • [Journal Article] Morphological and functional damage of retina caused by intravitreous indocyanine green in rat eyes.2004

    • Author(s)
      H.Enaida, et al.
    • Journal Title

      Graefes Archive for Clinical and Experimental Ophthalmology 240(3)

      Pages: 209-213

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Long-term histological and functional analysis for Simian Immunodeficiency Virus-based lentivirus vector-mediated intraocular gene transfer in adult rats.2003

    • Author(s)
      Ikeda Y, et al.
    • Journal Title

      Gene Therapy 10・14

      Pages: 1161-1169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The long-term effects of antiepileptic drugs on the visual system in rats : Electrophysiological and histopathological studies.2003

    • Author(s)
      Goto Y, et al.
    • Journal Title

      Clinical Neurophysiology 114・8

      Pages: 1395-1402

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Simian lentiviral vector-mediated retinal gene transfer of pigment epithelium-derived factor protects retinal degeneration and electrical defect in royal college of surgeons rats.2003

    • Author(s)
      Miyazaki M, et al.
    • Journal Title

      Gene Therapy 10・17

      Pages: 1503-1511

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Long-term histological and functional analysis for Simian Immunodeficiency Virus-based lentivirus vector-mediated intraocular gene transfer in adult rats.2003

    • Author(s)
      Y.Ikeda et al.
    • Journal Title

      Gene Therapy 10(14)

      Pages: 1161-1169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The long-term effects of antiepileptic drugs on the visual system in rats : Electrophysiological and histopathological studies.2003

    • Author(s)
      Y.Goto et al.
    • Journal Title

      Clinical Neurophysiology 114(8)

      Pages: 1395-1402

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Simian lentiviral vector-mediated retinal gene transfer of pigment epithelium-derived factor protects retinal degeneration and electrical defect in royal college of surgeons rats.2003

    • Author(s)
      M.Miyazaki et al.
    • Journal Title

      Gene Therapy 10(17)

      Pages: 1503-1511

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Morphological and functional damage of retina caused by intravitreous indocyanine green in rat eyes.2002

    • Author(s)
      Enaida H, et al.
    • Journal Title

      Graefes Archive for Clinical and Experimental Ophthalmology 240・3

      Pages: 209-213

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Critical role of photoreceptor apoptosis mediated via mitochondria-dependent pathways in the neuronal functional damage after experimental retinal detachment.2002

    • Author(s)
      Hisatomi T, et al.
    • Journal Title

      Current Eye Research 24・3

      Pages: 161-172

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Critical role of photoreceptor apoptosis mediated via mitochondria-dependent pathways in the neuronal functional damage after experimental retinal detachment.2002

    • Author(s)
      T.Hisatomi et al.
    • Journal Title

      Current Eye Research 24(3)

      Pages: 161-172

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Enaida H, et al.: "Morphological and functional damage of retina caused by intravitreous indocyanine green in rat eyes."Graefes Archive for Clinical and Experimental Ophthalmology. 240・3. 209-213 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hisatomi T, et al.: "Critical role of photoreceptor apoptosis mediated via mitochondria-dependent pathways in the neuronal functional damage after experimental retinal detachment."Current Eye Research. 24・3. 161-172 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikeda Y, et al.: "Long-term histological and functional analysis for Simian Immunodeficiency Virus-based lentivirus vector-mediated intraocular gene transfer in adult rats."Gene Therapy. 10・14. 1161-1169 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Goto Y, et al.: "The long-term effects of antiepileptic drugs on the visual system in rats : Electrophysiological and histopathological studies."Clinical Neurophysiology. 114・8. 1395-1402 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyazaki M, et al.: "Simian lentiviral vector-mediated retinal gene transfer of pigment epithelium-derived factor protects retinal degeneration and electrical defect in royal college of surgeons rats."Gene Therapy. 10・17. 1503-1511 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hisatomi T, et al.: "Critical role of photoreceptor apoptosis mediated via mitochondria-dependent pathways in the neuronal functional damage after experimental retinal detachment"Current Eye Research. 24. 161-172 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikeda Y, Goto Y, et al.: "Long-term histological and functional analysis for Simian Immunodeficiency Virus-based lentivirus vector-mediated intraocular gene transfer in adult rats"Gene Therapy. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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