| Project/Area Number |
14571714
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HASHIMOTO Ichiro The University of Tokushima, Department of Plastic & Reconstructive Surgery, Instructor, 大学院・ヘルスバイオサイエンス研究部, 助手 (70314870)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Hideki The University of Tokushima, Department of Plastic & Reconstructive Surgery, Professor, 大学院・ヘルスバイオサイエンス研究部, 教授 (90164235)
NAKAYA Yutaka The University of Tokushima, Department of Nutrition and Metabolism, Professor, 大学院・ヘルスバイオサイエンス研究部, 教授 (50136222)
TODA Maki The University of Tokushima, Department of Plastic & Reconstructive Surgery, Doctor, 医学部・歯学部附属病院, 医員(臨床)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Skin Microcirculation / Flap Necrosis / diabetes mellitus / Transcutaneous PO2 / Transcutaneous PCO2 |
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| Research Abstract |
The aim of this study is observation of character and failure of skin microcirculation in diabetes mellitus. Diabetes mellitus model of rabbits and normal rabbits as a control group were used. The following studies were performed.
1.Observation of skin microcirculation in the rabbit ear chamber in the diabetes mellitus rabbits and control rabbits.
2.Skin flap observations ; random pattern skin flap made on the back of rabbits and island flap made on the abdomen.
(1)Transcutaneous PO2 and PCO2 monitoring
(2)Dopplar flowmeter
(3)Skin necrosis
In the study of the random pattern skin flap, the difference of surviving area between the diabetes mellitus model and the control group was not significant. The reasons of this result are thought to be the blood glucose level, the duration of diabetes mellitus and the flap size. Serotonin (5-hydroxytryptamine) receptor antagonist showed inhibition effects to thrombus formation in arterioles and venules of the rabbit ear chamber by suppression of platelet aggregation. This drug inhibited the distal necrosis of the random pattern flap made on the back of rabbits. Histological findings of the flap revealed patent vessels in the deep dermis of the treated flap and occlusion of vessels in the deep dermis of the control group. These findings showed that flap survival effect of this agent is due to the suppression of the platelet aggregation in vessels of the deep dermis. The next study was histological analysis about skin flap survival effect by antagonist of leukocyte elastase. This agent showed the elongation effect of the skin flap damaged by ischemia reperfusion injury. Histological findings revealed that the infiltration of leukocyte into the skin in the control group was more than the infiltration in the treated goup.
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