| Project/Area Number |
14571965
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Meikai University |
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Principal Investigator |
TOKIYASU Yoshihiko Meikai Univ., Sch. of Dent., Asso. Professor, 歯学部, 助教授 (20163967)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | cementoblasts / enamel matrix protein / mineral nodule |
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| Research Abstract |
In order to design to predictable enzootics and replantation therapies in immature teeth, it is important to understand the responsiveness of cells within the local environment. The aim of this study was to determine the effect of enamel matrix protein (EMP) on cementoblasts. Methods : Osteocalcin (OC) promoter SV40 transgenic mice were used to obtain cementoblasts. Cementoblasts were exposed enamel matrix protein were evaluated for changes in : 1)proliferation over a 6-day period by cell counting ; 2)gene expression using Northern blot analysis ; and 3)biomineralization by von Kossa stain, in vitro and by preparing histological samples from implants retrieved from immunodeficient (SCID) mice, where cementoblasts were treated with enamel matrix protein prior to implantation. Results : EMP promoted proliferation of cementoblasts. EMP down-regulated osteocalcin transcripts in cementoblasts and up-regulated osteopontin gene expression. In vitro, EMP decreased cementoblasts -mediated biomineralization. In contrast, mineralization was noted in implants retrieved from SCID mice, where cells were pretreated with EMP. Conclusion : these results indicate that EMP can influence activities of cementoblasts.
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