The basic study of molecular mechanism to decide the size of teeth and jaws

• Project/Area Number: 14571966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 矯正・小児・社会系歯学
• Research Institution: Meikai University
• Principal Investigator: MANO Mikiko Meikai Univ., Dental Medicine, Lecture, 歯学部, 講師 (00333005)
• Project Period (FY): 2002 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,600,000); Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: epithelium tissue / IF5 / メカニカルストレス / 矯正力 / 顎の大きさ / 成長発育

Research Abstract

The IF5 gene was isolated by expression cloning from a cDNA library of mouse oocytes. It cDNA encodes a 250-amino acid protein and 1-137 amino acid region of it cDNA has 86.8% aligned the anti-proliferat domein. This gene was revealed to be a member of the BTG/TOB family, and this gene also was named as PTG4 or PC3B. The mRNA of IF5 was expressed in oocytes, tongue, palate, pharynx and trachea by RT-PCR.. The protein of IF5 was existed in epitherium cells of tongue, palate, pharynx, internal nares and trachea by IHC. Moreover, the protein levels of IF5 was rediuced in aging compare with 8 weeks and 1 year old mouse. Our findings suggest that IF5 may be one of the ageing related gene to regulate epithelial cell in oral and respilataly organ without lung.