• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Novel mechanisms for regulation by glutamate signals in expression of mitochondrial gene

Research Project

Project/Area Number 14572085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionSETSUNAN UNIVERSITY

Principal Investigator

OGITA Kiyokazu  Setsunan university, Pharmacology, Associate Professor, 薬学部, 助教授 (90169219)

Co-Investigator(Kenkyū-buntansha) YONEDA Yukio  Kanazawa University, Molecular Pharmacology, Professor, 大学院・自然科学研究科, 教授 (50094454)
Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsglutamate / kainate / N-methyl-D-aspartate / transcription factors / activator protein-1 / mitochondria / gel mobility shift assay / hippocampus / アクチベータープロティン-1 / グルタミン酸 / N-メチル-D-アスパラギン酸 / ゲル移動度シフト法
Research Abstract

A systemic administration of kainate dramatically enhanced AP-1 DNA binding in both mitochondrial and nuclear extracts of mouse cerebral cortex and hippocampus 1 h to 3 days later. Unlabeled AP-1 probe selectively competed for AP-1 DNA binding in mitochondrial extracts of cortex and hippocampus obtained from mice injected with kainate. Supershift and immunoblotting analyses revealed participation of c-Fos, Fos-B and Jun-B proteins in potentiation by kainate of mitochondrial AP-1 DNA binding in cortex and hippocampus. An immunohistochemical study demonstrated marked expression by kainate of c-Fos protein in the pyramidal and dentate granular layers, while an immunoelectron microscopic analysis showed localization of c-Fos protein within mitochondria, as well as nuclei, of the CA1 pyramidal and dentate granular cells in hippocampus obtained 2 h after the administration of kainate. There are 10 sites with sequences similar to the nuclear AP-1 site in the non-coding region. Of 10 pieces(termed as MT-1 to MT-10) of synthesized double-stranded oligonucleotides containing each mitochondrial AP-1-like site, MT-3,MT-4, and MT-9 were effective in inhibiting mitochondrial AP-1 DNA binding enhanced by kainate. Electrophoresis mobility shift analysis using radiolabeled MT-3 and MT-9 as probes demonstrated that marked enhancement was seen with binding of these 2 probes in hippocampal mitochondrial extracts prepared 2 to 6 h after kainate treatment. Unlabeled AP-1 probe was more potent than unlabeled MT-9 in inhibiting the mitochondrial MT-9 binding. Immunoprecipitation analysis using anti-c-Fos antibody demonstrated that c-Fos associated with mitochondrial genome in the hippocampal mitochondria prepared from kainate-treated animals. These results suggest that AP-1 complex expressed by in vivo treatment with kainate would bind to AP-1-like sites in the non-coding region of mitochondrial genome following translocation into the mitochondria in murine hippocampus.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Nobuyuki Kuramotoら: "Modulation of DNA binding of nuclear transcription factors with leucine-zipper motifs by particular endogenous polyamines in murine central and peripheral tissues."Brain Res.. 967. 170-180 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiyokazu Ogitaら: "In Vivo Neuroprotective Role of NMDA Receptors against Kainate-Induced Excitotoxicity in Murine Hippocampal Pyramidal Neurons."J.Neurochem.. 85. 1336-1346 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuyuki Kuramotoら: "A possible novel mechanism underlying temperature-dependent uptake of [^3H]spermidine in nuclear fractions of murine brain."Brain Res.. 981. 78-87 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiyokazu Ogitaら: "The transcription factor activator protein-1 expressed by kainate treatment can bind to the non-coding region of mitochondrial genome in murine hippocampus."J.Neurosci Res. 73. 794-802 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiji Inoueら: "Fos-B expression is required for polyamine-induced increase in nuclear activator protein-1 DNA binding in discrete structures of murine brain."J.Neurosci Res. 74. 199-209 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiji Inoueら: "Possible correlation between abilities of a variety of polyamines to increase activator protein-1 DNA binding and to inhibit [^3H]spermidine transport in nuclear fractions of murine brain."Brain Res.. 987. 126-130 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuyuki Kuramoto, Keiji Inoue, Keiko Gion, Katsura Takano, Katsumi Sakata, Kiyokazu Ogita, Yukio Yoneda: "Modulation of DNA binding of nuclear transcription factors with leucine-zipper motife by particular endogenous polyamines in murine central and peripheral tissues."Brain Res.. 967. 170-180 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiyokazu Ogita, Hiroaki Okuda, Yasuhiro Yamamoto, Norito Nishiyama, Yukio Yoneda: "In Vivo Neuroprotective Role of NMDA Receptors against Kainate-Induced Excitotoxicity in Murine Hippocampal Pyramidal Neurons."J.Neurochem.. 85. 1336-1346 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuyuki Kuramoto, Keiji Inoue, Katsura Takano, Hideo Taniura, Katsumi Sakata, Kiyokazu Ogita, Yukio Yoneda: "A possible novel mechanism underlying temperature-dependent uptake of [^3H]spermidine in nuclear fractions of murine brain."Brain Res.. 981. 78-84 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiyokazu Ogita, Yoshiaki Fujinami, Masahiro Kitano, Yukio Yoneda: "The transcription factor activator protein-1 expressed by kainate treatment can bind to the non-coding region of mitochondrial genome in murine hippocampus."J.Neurosci Res.. 73. 794-802 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiji Inoue, Nobuyuki Kuramoto, Chie Sugiyama, Hideo Taniura, Katsumi Sakata, Yoshiaki Fujinami, Kiyokazu Ogita, Yukio Yoneda: "Fos-B expression is required for polyamine-induced increase in nuclear activator protein-1 DNA binding in discrete structures of murine brain."J.Neurosci Res.. 15;74(2). 199-209 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Keiji Inoue, Nobuyuki Kuramoto, Katsura Takano, Hideo Taniura, Katsumi Sakata, Kiyokazu Ogita, Akira Shirahata, Yukio Yoneda: "Possible correlation between abilities of a variety of polyamines to increase activator protein-1 DNA binding and to inhibit [^3H]spermidine transport in nuclear fractions of murine brain,"Brain Res.Oct.. 10;987(1). 126-130 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiyokazu Ogita, Masaki Kubo, Norito Nishiyama, Mami Watanabe, Reiko Nagashima, Yukio Yoneda: "Enhanced binding activity of nuclear antioxidant-response element through possible formation of Nrf2/Fos-B complex after in vivo treatment with kainate in murine hippocampus."Neuropharmacology. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuyuki Kuramoto et al.: "Modulation of DNA binding of nuclear transcription factors with leucine-zipper motifs by particular endogenous polyamines in murine central and peripheral tissues."Brain Res.. 467. 170-180 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kiyokazu Ogita et al.: "In Vivo Neuroprotective Role of NMDA Receptors against Kainate-Induced Excitotoxicity in Murine Hippocampal Pyramidal Neurons."J.Neurochem. 85. 1336-1346 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nobuyuki Kuramoto et al.: "A possible novel mechanism underlying temperature-dependent uptake of [^3H]spermidine in nuclear fractions of murine brain."Brain Res.. 981. 78-84 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keiji Inoue et al.: "Fos-B expression is required for polyamine-induced increase in nuclear activator protein-1 DNA binding in discrete structures of murine brain."J.Neurosci.Res.. 74. 199-209 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kiyokazu Ogita et al.: "The transcription factor activator protein-1 expressed by kainate treatment can bind to the non-coding region of mitochondrial genome in murine hippocampus."J.Neurosci.Res.. 73. 794-802 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Keij Inoue: "Possible correlation between abilities of a variety of polyamines to increase activator protein-1 DNA binding and to inhibit [^3H]spermidine transport in nuclear fractions of murine brain."Brain Res.. 987. 126-130 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kiyokazu Ogita: "Localization of activator protein-1 complex with DNA binding activity in mitochondria of murine brain following in vivo treatment with kainate"J. Neurosci.. 22・7. 2561-2570 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 荻田喜代一: "神経細胞障害に対するN-methyl-D-aspartic acidの防御作用"日本神経精神薬理学雑誌. 22. 153-158 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kiyokazu Ogita: "In Vivo Neuroprotective Role of NMDA Receptors against Kainate-Induced Excitotoxicity in Murine Hippocampal Pyramidal Neurons"J. Neurochem.. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] 荻田喜代一: "グルタミン酸神経毒性とミトコンドリア遺伝子"Clinical Neuroscience. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Yukio Yoneda: "Constitutive expression of heterologous N-methy-D-aspartate receptor subunits in rat adrenal medulla"J. Neurosci. Res.. 68・1. 36-45 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yukio Yoneda: "Modulation of DNA binding of nuclear transcription factors with leucine-zipper motifs by particular endogenous polyamines in murine central and peripheral tissues"Brain Res.. (印刷中).

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2002-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi