|Budget Amount *help
¥3,100,000 (Direct Cost : ¥3,100,000)
Fiscal Year 2003 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 2002 : ¥2,600,000 (Direct Cost : ¥2,600,000)
How dried squids will change the physicochemical properties and microstructure under softening treatment.
Abstract : The two types of squids, dried at 4 degrees centigrade (A) and 40 degrees c (B), were softened in water and then in alkaline solution, and their physiochemical and structural changes were examined. A significantly higher wet weight was observed on A compared with B. During the softening treatment, the B group showed far higher figures in both of the rupture stress and energy than the A group. The sodium dodecyl sulphate polyactylamide slab gel electrophoresis (SDS-PAGE) pattern of A was almost same as that of raw squids, while B's SDS-PAGE exhibited many fragments of lower molecular weight. After having been soaked in distilled water, B's SDS-PAGE pattern did not change remarkably but its muscle fiber bundles were observed by histological analysis by light microscopy. Group B showed higher water permeation on the muscle fibers when alkaline softened. Comparative tests were conducted by time and temperature on Groups A and B, each soaked in distlled water. Group A produced AMP and IMP contents more in the order of 60, 20, 5 and 95 degrees centigade. In case of Group B, those contents more in tile order of 60, 95, 20 and 5 degrees centigrade.