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Purification and Functional Analysis of Thermo-sensory channels

Research Project

Project/Area Number 14580675
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

NAKAOKA Yasuo  NAKAOKA,Yasuo, 生命機能研究科, 助教授 (90029562)

Project Period (FY) 2002 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsThermosensory / Lonic channels / Paramecium / Membrane fluidity / Laurdan / Latty acids / 温度感覚 / 蛋白精製 / リゾチーム
Research Abstract

From the cell membrane of Paramecium, we tried to isolate and purify the channel proteins whose activity is sensitive to temperature change. Although the purification was, yet incomplete, we found some chemicals that inhibit specifically thermosensory response of Paramecium. The inhibitors were lysozyme, ruthenium-red etc. These inhibitors are polyvalent cations in the neutral solution. From such nature of the inhibitors, following two possibilities are considered for the inhibitory mechanism ; (1) Inhibitors directly bind to the thermosensory channels to block ionic current. (2) Inhibitors indirectly bind to the negatively charged lipids surrounding thermosensory channels, which alters the membrane fluidity and inhibits the activity of channels. In order to test the possibility (2), we constructed the fluorescence microscope system that can measure the membrane fluidity of the living cells stained with fluorescent probe, laurdan. From P. aurelia group, we have already selected each species of high and low thermotolerance. Comparing the membrane fatty acid contents between these species, the high thermotolerant species have a decreased ratio of unsaturated to saturated fatty acids than the low thermotolerant species. Measurements of the membrane fluidity using the fluorescence microscope system showed that the high thermotolerant species had a decreased membrane fluidity from the low thermotolerant species. We will measure rapid changes of membrane fluidity in response to temperature change.

Report

(3 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Y.Nakaoka: "Orientation of paramecium swimming in a DC magnetic field."Bioelectromagnetics. 23. 607-613 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Iwamoto: "External GTP binding and induction of cell division in starved Tetrahymena thermophila"Europ.J.Cell Biol. 81. 517-524 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Takeuchi: "Diamagnetic Orientation of Bull Sperms and Related Materials in Static Magnetic Fields"J.Phys.Soc.Japan. 71. 363-368 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Emura: "Analysis of anisotropic diamagnetic susceptibility of a bull sperm"Bioelectromagnetics. 24. 347-355 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中岡保夫: "原生生物における電磁場の影響"物性研究. 82. 77-81 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Iwamoto: "Cell division induced by mechanical stimulation in starved Tetrahymena thermophila"Cell Biol International. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakaoka, Y, Takeda, R. Shimizu, K.: "Orientation of Paramecium swimming in a DC magnetic field."Bioelectromagnetics. 23. 607-613 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iwamoto, M.Nakaoka, Y.: "External GTP binding and induction of cell division in starved Tetrahymena thermophila."Europ.J.Cell Biol.. 81. 517-524 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takeuchi, T., Nakaoka, Y., Emura, R, Higashi, T.: "Diamagnetic orientation of bull sperms and related materials in static magnetic fields."J.Phys.Soc.Japan. 71. 363-368 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Emura, R., Takeuchi, T., Nakaoka, Y.Higashi, T.: "Analysis of anisotropic diamagnetic susceptability of a bull sperm."Bioelectromagnetics. 24. 347-355 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakaoka, Y.: "Effects of electromagnetic fields on protozoa."Bussei Kenkyu. 82. 77-81 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iwamoto, M., Sugai, T.Nakaoka, Y.: "Cell division induced by mechanical stimulation in starved Tetrahymena therm op hila: cell cycle without synthesis of macronuclear DNA."Cell Biology International. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Runa Emura: "Analysis of Anisotropic Diamagnetic Susceptibility of a Bull Sperm"Bioelectromagnetics. 24. 347-355 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中岡保夫: "原生生物における電磁場の影響"物性研究. 82・1. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iwamoto, M.: "External GTP binding and induction of cell division in starved Tetrahymena thermophila"Eur. J. Cell Biol.. 81・9. 517-524 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakaoka, Y.: "Orientation of Paramecium Swimming in a DC magnetic field"Bioelectromagnetics. 23・8. 607-613 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimizu, K.: "High density 60Hz magnetic field has no effects on mutagenecity and growth of the budding yeast"Rad. Safety Manag.. 1-1. 17-20 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeuchi, T.: "Diamagnetic orientation of bull spermo and related materials in static magnetic fields"J. Phys. Soc. Japan. 71. 363-368 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Emura, R.: "Analysis of anisotropic diamagnetic susceptibility of a bull sperm"Bioelectromagnetics. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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