Pre-B lymphoma genesis induced by endogeneous retrovirus integration
| Project/Area Number |
14580799
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TSURUYAMA Tatsuaki Kyoto University, Department of Pathology and Biology of Diseases, Instructor, 医学研究科, 助手 (00303842)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Yoshihiro Kyoto University, Department of Pathology and Biology of Diseases, Lecturer, 医学研究科, 講師 (30252464)
HIAI Hiroshi Shiga Medical Center for Adults, President, 医学研究科, 教授 (10073131)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | genetics / retrovirus / cancer / signal transduction / pathology / ウイルス / レトロウィルス / リンパ腫 / STAT5A / プレB細胞 |
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| Research Abstract |
We found that the second intron of stat5a, Stat5b, c-myc. evi3/svi2 were the common integration sites of the endogenous ecotropic murine leukemia virus in early pre-B lymphomas in SL/Kh mice. The high expression of stat5a induced by the integration and activation accelerated the expression. Transfection of the constitutively active stat5a mutant cDNA, but not of the wild-type cDNA, to the bone marrow cells induced colony formation of pre-B cells in a methylcellulose medium and escaped from dependence on IL-7. Such growth was dependent on a genetic factor in the SL/Kh strain. Consitutively high expression of Stat5a either by retrovirus integration or by transfection of active mutant cDNA can be lymphomagenic to early pre-B cells in collaboration with a certain genetic background factor of mice. The phenotype analysis revealed that lymphoma cells with c-myc, or evi3 integration was maturer than lymphoma cells with srat5a integration, suggesting that activated genes by integration may influence the phenotype of lymphoma cells.
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Report
(3 results)
Research Products
(10 results)
