| Project/Area Number |
14580813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ICHINOSE Shizuko Tokyo Medical and Dental University, Instrumental Analysis Research Center for Life Science, Lecturer, 機器分析センター, 助手 (60014156)
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| Co-Investigator(Kenkyū-buntansha) |
MUNETA Takeshi Tokyo Medical and Dental University, Graduate School, Section of Orthopaedic Surgery, Professor, 大学院・医歯学総合研究科, 教授 (50190864)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | Human mesenchymal stem cells / Chondrogenesis induction / Alginate bead culture / Pellet culture / Implantation / Cartilage formation / ヒト骨髄由来間葉系幹細胞 / アルジネート培養 / TGFβ-3 |
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| Research Abstract |
Human mesenchymal stem cells (hMSCs) have the potential to differentiate into the lineages of mesenchymal tissues. The aim of this study Is to clarify the detailed process of differentiation In hMSCs. We examined cartilage formation of human mesenchymal stem cells using three dimentional culture and implantation.
Three dimentional culture -alginate bead culture-: hMSCs were maintained in alginate beads. We examined them by light microscope, transmission electron microscope and immunocryo-ultramicrotomy. hMSCs produced collagen type II, X, proteoglycan and osteocalcin at day 4 in the case of alginate bead culture. Thereafter electron-dense particles, composed of hydroxyapatite, appeared.
Three dimentional culture -pellet culture-: In the case of pellet culture, hMSCs were connected with junctional complex at day 1. At day 7, hMSCs produced collagen type I, II, X and proteoglycan. During day 14-21, the expression of collagen type II decreased.
Implantation of MSO-derived cartilages into the rabbit cartilage defect.: We cultured MSCs as a micromass in chondrogenesis medium for 14 days and differentiated MSCs into cartilage in vitro. Thereafter, MSC-derived cartilages were press fitted into the rabbit cartilage defects. Eight weeks after implantation, we observed the cartilage defects were repaired.
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