Apoptosis of helper T cells via the costimulatory molecules OX40 stimulated by gp34
| Project/Area Number |
14599010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
細胞死(アポトーシス)
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
TANAKA Yuetsu University of the Ryukyus, Faculty of Medicine, Professor, 医学部, 教授 (30163588)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | OX40 / apoptosis / TNF-R / HIV-1 / CD4 / OX40 |
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| Research Abstract |
OX40 belongs to TNF receptor(TNF-R) super family and plays an important role in survival of antigen-pecific CD4^+ T cells. We have previously reported that the individual stimulation of OX40 or TNF-R by respective ligands of HIV-1-latently infected ACH-2/OX40 cells results in the activation of HIV-1 production via the NF-kB pathway. In the present study, we examined the effect of dual stimulation by OX40L and TNF on HIV-1 activation. In contrast to individual stimulation, HIV-1 production was severely interrupted by the dual stimulation, which was reversed by anti-OX40L antibody. Similar effects were also observed in other OX40-expressing, HIV-1 productively-chronically-and acutely-infected cells, Molt4/IIIB, U1 and Molt4, respectively. Microscopic observation of cell morphology and Annexin-V staining showed that the double-stimulated cells rapidly became apoptotic. The dual stimulation also induced cell death in T cell lines that were not infected with HIV-1(Molt-4/OX40,CEM/OX40 and Jurkat/OX40). In contrast, two promonocytic cell lines(U937/OX40 and THP-1/OX40) were relatively resistant, and a B cell line(BJAB/OX40) was completely resistant to the dual stimulation. A broad caspase inhibitor, Z-VAD-FMK, significantly reduced the death of Molt-4/OX40 cells. These results suggest a new biological role of OX40 in controlling not only HIV-1 production but also the fate of CD4^+ T cells in infection and inflammatory environments.
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Report
(3 results)
Research Products
(7 results)
