• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

二本鎖DNA切断により誘導される複製プログラム変動のメカニズム

Research Project

Project/Area Number 14F03511
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Architectural history/Design
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

正井 久雄  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 副所長 (40229349)

Co-Investigator(Kenkyū-buntansha) LAI MONG SING  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 外国人特別研究員
LAI Mong Sing  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 外国人特別研究員
Project Period (FY) 2014-04-25 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2015: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsRif1 / yKu70 / Double-strand DNA break / DNA replication / Replication origin / Late and dormant origin / Telomere / Budding yeast / Double-strand break / Dormant origins
Outline of Annual Research Achievements

Various types of DNA damage can induce activation of dormant replication origins. Previous studies showed that a single and irrepairable double-strand break (DSB) in the proximity of a dormant replication origin is able to promote origin firing, likely to rescue replication of the broken chromosome fragment (Doksani et al., Cell 2009). In this study, We aim at elucidating the mechanisms leading to dormant origin firing following DSB formation.
Here we showed that DNA damage checkpoints do not counteract DSB-induced dormant origin firing but the dormant origin firing activities are counteracted by histone acetylation state. We also identified telomere-related proteins, Rif1 and Ku complex, as potential regulators of dormant origins even in the absence of DSB. We found that Rif1 and yKu70 bind preferentially at regions between dormant origins and DSB site but not at the other side of the DSB site. The binding profiles of Rif1 and yKu70 at those regions are not affected by DSB formation. Besides, we found that absence of yKu70 prevents Rif1 from binding at regions between dormant origins and DSB site. Our results indicate an important role of Rif1 and yKu70 in controlling dormant origin activity near to DSB site.

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2016 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 8 results,  Acknowledgement Compliant: 8 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 7 results) Book (2 results) Remarks (1 results)

  • [Journal Article] CDC28 phosphorylates Cac1p and regulates the association of Chromatin Assembly Factor I with chromatin.2015

    • Author(s)
      Jeffery, D., Kakusho, N., You, Z., Gharib, M., Wyse, B., Drury, E., Weinreich, M., Thibault, P. Verreault, A., Masai, H. and Yankulov, K.
    • Journal Title

      Cell Cycle

      Volume: 14 Pages: 74-85

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Definition of the transcription factor TdIF1 consensus binding sequence through genome-wide mapping of its binding sites.2015

    • Author(s)
      Kotaro Koiwai, Takashi Kubota, Nobuhisa Watanabe, Katsutoshi Hori, Osamu Koiwai and Hisao Masai
    • Journal Title

      Genes to Cells

      Volume: 20 Issue: 3 Pages: 242-254

    • DOI

      10.1111/gtc.12216

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] NCOA4 Transcriptional Coactivator Inhibits Activation of DNA Replication Origins.2014

    • Author(s)
      Bellelli, R., Castellone, M.D., Guida, T., Limongello, R., Dathan, N.A., Merolla, F., Cirafici, A.M., Affuso, A., Masai, H., Costanzo, V., Grieco, D., Fusco, A., Santoro, M., and Carlomagno, F.
    • Journal Title

      Mol. Cell

      Volume: 55 Issue: 1 Pages: 123-137

    • DOI

      10.1016/j.molcel.2014.04.031

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation and roles of Cdc7 kinase under replication stress.2014

    • Author(s)
      Yamada, M., Masai, H., and Bartek, J.
    • Journal Title

      Cell Cycle

      Volume: 13 Issue: 12 Pages: 1859-1866

    • DOI

      10.4161/cc.29251

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cell cycle synchronization and flow cytometry analysis of mammalian cell.2014

    • Author(s)
      Yoshizawa-Sugata, N. and Masai, H.
    • Journal Title

      Methods in Molecular Biology

      Volume: 1170 Pages: 279-293

    • DOI

      10.1007/978-1-4939-0888-2_13

    • ISBN
      9781493908875, 9781493908882
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ATM in prevention of genomic instability.2014

    • Author(s)
      Hisao Masai
    • Journal Title

      Cell Cycle

      Volume: 13 Issue: 6 Pages: 882-883

    • DOI

      10.4161/cc.28216

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Temporal and spatial regulation of eukaryotic DNA replication: from regulated initiation to genome-scale timing program.2014

    • Author(s)
      Renard-Guillet, C., Kanoh, Y., Shirahige, K., and Masai, H.
    • Journal Title

      Seminars in Cell & Developmental Biology,

      Volume: 30 Pages: 110-120

    • DOI

      10.1016/j.semcdb.2014.04.014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] H2B Mono-ubiquitylation Facilitates Fork Stalling and Recovery during Replication Stress by Coordinating Rad53 Activation and Chromatin Assembly2014

    • Author(s)
      Chia-Yeh Lin, Meng-Ying Wu, Sophie Gay, Lisette Marjavaara, Mong Sing Lai, Wei-Chun Hsiao, Shih-Hsun Hung, Hsin-Yi Tseng, Duncan Edward Wright, Chen-Yi Wang, Guoo-Shyng W. Hsu, Didier Devys, Andrei Chabes, and Cheng-Fu Kao
    • Journal Title

      PLoS Genetics

      Volume: 10 Issue: 10 Pages: 1-17

    • DOI

      10.1371/journal.pgen.1004667

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Replicon dynamics in response to double-strand break formation2016

    • Author(s)
      MongSing Lai
    • Organizer
      The 10th 3R Symposium
    • Place of Presentation
      ホテル一畑(島根県松江市)
    • Year and Date
      2016-11-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生物種を超えた統一像と多様性 (Conserved mechanisms and diversity of genome DNA replication: from bacteria to human)」イントロダクション2014

    • Author(s)
      正井 久雄
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Mechanisms controlling replicon dynamics following double-strand break formation2014

    • Author(s)
      Lai Mong Sing
    • Organizer
      The 9th 3R Symposium
    • Place of Presentation
      御殿場高原リゾート(静岡県)
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Regulation of mammalian Mcm helicase complexes2014

    • Author(s)
      Hisao Masai, You Zhiying, Koji L. Ode, Naoko Kakusho, Rino Fukatsu, and Haruhiko Takisawa
    • Organizer
      第87回日本生化学会大会シンポジウム「ゲノムを支えるDNAヘリカーゼの動態を探求する新たな研究展開」
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 染色体DNA複製プログラムの制御機構2014

    • Author(s)
      正井 久雄
    • Organizer
      大阪大学蛋白質研究所セミナー「染色体伝承の分子背景:複製から染色体分離まで」
    • Place of Presentation
      大阪大学(大阪)
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Regulation of DNA replication program in fission yeast and human cells2014

    • Author(s)
      Hisao Masai
    • Organizer
      Marco Foiani(Italy, Milan)教授の招聘により IFOM Foundation – F.I.R.C. Institute of Molecular Oncology Foundationで講演
    • Place of Presentation
      Italia, Milan
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Mrc1/Claspinの分子内相互作用による活性制御および新規機能2014

    • Author(s)
      正井 久雄
    • Organizer
      九州大学 大学院薬学研究院 招聘研究セミナー
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2014-08-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Regulation of replication program in fission yeast and human cells.2014

    • Author(s)
      Hisao Masai
    • Organizer
      Peking University(特別招聘講演)
    • Place of Presentation
      Peking University(中国)
    • Year and Date
      2014-05-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] “DNA replication: From Old Principles to New Discovery” in “Advances in Experimental Medicine and Biology”2017

    • Author(s)
      Kenji Moriyama, Mong Sing Lai, and Hisao Masai
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] DNA Replication, Recombination and Repair: Molecular Mechanisms and Pathology2015

    • Author(s)
      Motoshi Hayano, Seiji Matsumoto and Hisao Masai
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Remarks] 公益財団法人東京都医学総合研究所ゲノム動態プロジェクト

    • URL

      http://www.igakuken.or.jp/genome/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-01-22   Modified: 2024-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi