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月周同調産卵におけるキスペプチン-GnRH系の制御機構と機能の解明

Research Project

Project/Area Number 14F04068
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Morphology/Structure
Research InstitutionNiigata University

Principal Investigator

安東 宏徳  新潟大学, 自然科学系, 教授 (60221743)

Co-Investigator(Kenkyū-buntansha) SHAHJAHAN MD.  新潟大学, 自然科学系, 外国人特別研究員
SHAHJAHAN Md.  新潟大学, 自然科学系, 外国人特別研究員
Project Period (FY) 2014-04-25 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2016: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2015: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2014: ¥400,000 (Direct Cost: ¥400,000)
Keywordsキスペプチン / GnRH / メラトニン / 温度感受性TRPチャンネル / 脳・神経 / 月周リズム / 生殖リズム / 神経ホルモン / クサフグ / 月周産卵
Outline of Annual Research Achievements

本研究では、半月周性の産卵リズムをもつクサフグを研究モデルとして、月周産卵リズムの形成におけるKiss-GnRH系の機能とその分子機構を明らかにする。これまでの研究から、GnRH2、Kiss、Kiss受容体の遺伝子発現が同期して日周および概日変動し、これらの生殖調節神経ホルモンの発現が松果体にある概日時計と松果体から分泌されるメラトニンによって調節される可能性が示された。本研究では、KissニューロンとGnRHニューロンの機能形態学的相互関係を明らかにし、それを基盤として、メラトニンや弱光、さらに水温によるKiss-GnRH系の機能の調節機構を明らかにする。平成28年度では、次の1)と2)の解析を行った。
1)予定していた弱光暴露実験は、魚が採集できず実施できなかった。月光によるKiss-GnRH系の調節に関する実験として、Kiss-GnRH系の遺伝子の発現の月周変動を検討した。産卵期のクサフグの間脳で、kiss2とkiss2r、gnrh1の発現量は、夜間において月齢15日(満月)に発現量が上昇した。一方、gnrh2は月齢10日と25日に発現量が上昇した。gnrh3は、月齢に伴った変化を示さなかった。
2)KissによるKiss-GnRH系の調節を検討した。未成熟なクサフグ1歳魚、産卵場で採集した成熟個体共に、Kiss2投与によりkiss2rとgnrh1の発現量は用量依存的に増加した。一方、gnrh2とgnrh3の発現量は変化が見られなかった。下垂体においては、fshbとlhbの発現量がKiss2投与によって増加した。
これらの結果から、Kiss2は、Kiss受容体を介してGnRH1の発現を刺激し、FSHとLHの合成量を増加させることが明らかになった。また、このKiss-GnRH1系の月齢に伴った機能亢進が満月の夜の産卵を引き起こすことが示唆された。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (46 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (22 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 19 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (17 results) (of which Int'l Joint Research: 5 results,  Invited: 4 results) Book (3 results) Remarks (2 results)

  • [Int'l Joint Research] Bangladesh Agricultural University(バングラデシュ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Bangladesh Agricultural University(バングラデシュ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Temperature affects sexual maturation through the control of kisspeptin, kisspeptin receptor, GnRH and GTH subunit gene expression in the grass puffer during the spawning season.2017

    • Author(s)
      Shahjahan, Md., Kitahashi, T., Ando, H.
    • Journal Title

      Gen. Comp. Endocrinol.

      Volume: 243 Pages: 138-145

    • DOI

      10.1016/j.ygcen.2016.11.012

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Pollution of radiocesium and radiosilver in wharf roach (Ligia sp.) by the Fukushima Dai-ichi Nuclear Power Plant accident.2017

    • Author(s)
      Qiu, X., Undap, S.L., Honda, M., Sekiguchi, T., Suzuki, N., Shimasaki, Y. Ando, H., Sato-Okoshi, W., Wada, T., Sunobe, T., Takeda, S., Munehara, H., Yokoyama, H., Momoshima, N., Oshima, Y.
    • Journal Title

      J. Radioanal. Nucl. Chem.

      Volume: 311 Issue: 1 Pages: 121-126

    • DOI

      10.1007/s10967-016-4879-1

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of Sumithion on Growth and Production of Phytoplankton and Zooplankton in Aquaculture Pond.2017

    • Author(s)
      Hossain MI, Rahman MS, Amin AKMR, Ahmed SI, Shahjahan M.
    • Journal Title

      Iranian Journal of Fisheries Sciences

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in hematological parameters and gill morphology in common carp exposed to sub-lethal concentrations of Malathion.2016

    • Author(s)
      Sharmin S, Salam MA, Haque F, Islam MS, Shahjahan M.
    • Journal Title

      Asian Journal of Medical and Biological Research

      Volume: 2 Issue: 3 Pages: 370-378

    • DOI

      10.3329/ajmbr.v2i3.30106

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in hepatosomatic index and histoarchitecture of liver in common carp exposed to organophosphate insecticide sumithion.2016

    • Author(s)
      Hossain S, Miah MI, Islam MS, Shahjahan M.
    • Journal Title

      Asian Journal of Medical and Biological Research

      Volume: 2 Issue: 2 Pages: 164-170

    • DOI

      10.3329/ajmbr.v2i2.29006

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alteration of blood glucose and hemoglobin levels in zebrafish exposed to sumithion.2016

    • Author(s)
      Ahmed SI, Zahangir MM, Haque F, Ahmmed MK, Shahjahan M.
    • Journal Title

      Progressive Agriculture

      Volume: 27 Issue: 2 Pages: 216-221

    • DOI

      10.3329/pa.v27i2.29333

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LPXRFamide peptide stimulates growth hormone and prolactin gene expression during the spawning period in the grass puffer, a semi-lunar synchronized spawner.2016

    • Author(s)
      Shahjahan, M., Doi, H., Ando, H.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 227 Pages: 77-83

    • DOI

      10.1016/j.ygcen.2015.09.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] クサフグの松果体におけるメラトニン受容体遺伝子のウルトラディアン発現2016

    • Author(s)
      安東宏徳
    • Journal Title

      Comparative Endocrinology

      Volume: 42 Issue: 157 Pages: 7-8

    • DOI

      10.5983/nl2008jsce.42.7

    • NAID

      130005128693

    • ISSN
      1882-6636, 1882-6644
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impacts of organophosphate pesticide, sumithion on water quality and benthic invertebrates in aquaculture ponds.2016

    • Author(s)
      Uddin M.H., Shahjahan M., Amin A.K.M.R., Haque M.M., Islam M.A., Azim M.E.
    • Journal Title

      Aquaculture Reports

      Volume: 3 Pages: 88-92

    • DOI

      10.1016/j.aqrep.2016.01.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cyclic variations of gonad development of an air-breathing fish, Channa striata in the lentic and lotic environments.2016

    • Author(s)
      Al Mahmud N., Rahman H.M.H., Mostakim G.M., Khan M.G.Q., Shahjahan M., Lucky N.S., Islam M.S.
    • Journal Title

      Fisheries and Aquatic Sciences

      Volume: 19 Issue: 1 Pages: 5-5

    • DOI

      10.1186/s41240-016-0005-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toxicity of Organophosphorous Pesticide Sumithion on Larval Stages of Stinging Catfish Heteropneustes fossilis.2016

    • Author(s)
      Shahjahan M., Kabir M.F., Sumon K.A., Bhowmik L.R., Rashid H.
    • Journal Title

      Chinese Journal of Oceanography and Limnology

      Volume: 印刷中 Issue: 1 Pages: 109-114

    • DOI

      10.1007/s00343-016-5173-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クサフグの半月周性産卵回遊行動とホルモン2015

    • Author(s)
      安東宏徳
    • Journal Title

      海洋と生物

      Volume: 37 Pages: 569-575

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Impacts of Sumithion on Blood Glucose and Some Hematological Parameters in Common Carp.2015

    • Author(s)
      Hossain S., Khatun M.H., Rahman M.K., Shahjahan M.
    • Journal Title

      International Journal of Environment

      Volume: 5 Pages: 8-13

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Reproductive biology of great snakehead Channa marulius from Sylhet basin in the North East Bangladesh.2015

    • Author(s)
      Siddiquee A., Rashid H., Islam M.A., Ahmed K.K.U., Shahjahan M.
    • Journal Title

      Journal of Fisheries and Aquatic Sciences

      Volume: 10 Issue: 4 Pages: 294-299

    • DOI

      10.3923/jfas.2015.294.299

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Sub-lethal Doses of an Organophosphorus Insecticide Sumithion on Some Hematological Parameters in Common Carp, Cyprinus carpio.2015

    • Author(s)
      Salam M.A., Shahjahan M., Sharmin S., Haque F., Rahman M.K.
    • Journal Title

      Pakistan Journal of Zoology

      Volume: 47 Pages: 1487-1491

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histopathological Changes in Liver and Kidney of Common Carp Exposed to Sub-lethal Doses of Malathion.2015

    • Author(s)
      Sharmin S., Shahjahan M., Hossain M.A., Haque M.A., Rashid H.
    • Journal Title

      Pakistan Journal of Zoology

      Volume: 47 Pages: 1495-1498

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic differentiation of wild and hatchery populations of Indian major carp in Bangladesh.2015

    • Author(s)
      Sharker M.R., Siddik M.A.B., Nahar A., Shahjahan M., Faroque A.A.
    • Journal Title

      Journal of Environmental Biology

      Volume: 36 Pages: 1223-1227

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histo-architectural changes of intestinal morphology in Zebra fish (Danio rerio) exposed to Sumithion.2015

    • Author(s)
      Ahmed S.I., Ahmmed M.K., Ghosh S.K., Islam M.M., Shahjahan M.
    • Journal Title

      Research in Agriculture, Livestock and Fisheries

      Volume: 2 Issue: 3 Pages: 499-506

    • DOI

      10.3329/ralf.v2i3.26174

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Culture of Chlorella ellipsoidea in different inexpensive medium and used as food for production of rotifers Brachionus calyciflorus.2015

    • Author(s)
      Akter S., Shahjahan M., Hossain S., Rahman M.S.
    • Journal Title

      Iranian Journal of Fisheries Sciences

      Volume: 15 Pages: 558-566

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential expression patterns of PQRFamide peptide and its two receptor genes in the brain and pituitary of grass puffer during the reproductive cycle.2015

    • Author(s)
      Shahjahan, M., Doi, H., Ando, H.
    • Journal Title

      General and Comparative Endocrinology

      Volume: 210 Pages: 152-160

    • DOI

      10.1016/j.ygcen.2014.07.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ultradian oscillation in expression of four melatonin receptor subtype genes in the pineal gland of the grass puffer, a semilunar-synchronized spawner, under constant darkness2015

    • Author(s)
      Ikegami T, Maruyama Y, Doi H, Hattori A, Ando H
    • Journal Title

      Frontiers in Neuroscience

      Volume: 9 Pages: 1-10

    • DOI

      10.3389/fnins.2015.00009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diurnal and circadian oscillations in expression of kisspeptin, kisspeptin receptor and gonadotrophin-releasing hormone 2 genes in the grass puffer, a semilunar-synchronised spawner2014

    • Author(s)
      Ando H, Ogawa S, Shahjahan Md, Ikegami T, Doi H, Hattori A, Parhar I
    • Journal Title

      Journal of Neuroendocrinology

      Volume: 26 Issue: 7 Pages: 459-467

    • DOI

      10.1111/jne.12165

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] クサフグの半月周性産卵リズム:日本海 vs 太平洋2017

    • Author(s)
      安東宏徳
    • Organizer
      形の科学研究センター2016年第2回セミナー
    • Place of Presentation
      東洋大学熱海研修センター(静岡県・熱海市)
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] クサフグ間脳におけるウルトラディアン発現遺伝子の探索2016

    • Author(s)
      山田佑紀,渡邊太朗,土井啓行,兵藤晋,安東宏徳
    • Organizer
      第41回日本比較内分泌学会大会
    • Place of Presentation
      北里大学(神奈川県・相模原市)
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 佐渡におけるクサフグの半月周性産卵リズムの解析-雄はリズムをもたないが、雌はリズムをもつ?2016

    • Author(s)
      佐藤鴻志郎,山田佑紀,Md. Shahjahan,加藤陽一郎,飯田碧,北橋隆史,安東宏徳
    • Organizer
      第41回日本比較内分泌学会大会
    • Place of Presentation
      北里大学(神奈川県・相模原市)
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Thermosensitive expression of transient receptor potential genes, TRPV1a and TRPA1, in the brain of grass puffer during the spawning season.2016

    • Author(s)
      Md. Shahjahan, Hironori Ando
    • Organizer
      87th meeting of the Zoological Society of Japan
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Circadian and lunar age-dependent oscillations in expression of GnIH, kisspeptin and their receptor genes in the grass puffer, a semilunar spawner.2016

    • Author(s)
      Hironori Ando, Md. Shahjahan
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology and the 87th meeting of the Zoological Society of Japan
    • Place of Presentation
      沖縄科学技術大学院大学(沖縄県・国頭郡)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Thermoregulation of hypothalamic reproductive neurohormone gene expression in the grass puffer during the spawning season.2016

    • Author(s)
      Md. Shahjahan, Takashi Kitahashi, Hironori Ando
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology and the 87th meeting of the Zoological Society of Japan
    • Place of Presentation
      沖縄科学技術大学院大学(沖縄県・国頭郡)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of daily and lunar-synchronized reproductive rhythms by GnIH and kisspeptin.2016

    • Author(s)
      Hironori Ando, Md. Shahjahan
    • Organizer
      8th Congress of the Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      ソウル(大韓民国)
    • Year and Date
      2016-06-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] In vivo melatonin treatment modulates expression of kisspeptin, kisspeptin receptor and GnRH genes via melatonin receptor in the grass puffer.2016

    • Author(s)
      Md. Shahjahan, Atsuhiko Hattori, Hironori Ando
    • Organizer
      8th Congress of the Asia and Oceania Society for Comparative Endocrinology
    • Place of Presentation
      ソウル(大韓民国)
    • Year and Date
      2016-06-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] メラトニンによるキスペプチン,キスペプチン受容体およびメラトニン受容体遺伝子の発現誘導2016

    • Author(s)
      安東宏徳,Md. Shahjahan,土井啓行,服部淳彦
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Lunar-synchronized expressions of cryptochrome genes in the diencephalon of grass puffer.2015

    • Author(s)
      Takehiro Ishikawa, Hiroyuki Doi, Hironori Ando
    • Organizer
      第40回日本比較内分泌学会大会
    • Place of Presentation
      JMSアステールプラザ(広島)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Lunar age-dependent variation in expressions of LPXRFamide peptide and its receptor genes in the grass puffer.2015

    • Author(s)
      Yuki Yamada, Md. Shahjahan, Hiroyuki Doi, Hironori Ando
    • Organizer
      第40回日本比較内分泌学会大会
    • Place of Presentation
      JMSアステールプラザ(広島)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Temperature affects kisspeptin and GnRH gene expression in the grass puffer during the spawning season.2015

    • Author(s)
      Md. Shahjahan, Hironori Ando
    • Organizer
      第40回日本比較内分泌学会大会
    • Place of Presentation
      JMSアステールプラザ(広島)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Lunar-synchronized expression of kisspeptin, kisspeptin receptor and GnRH genes in grass puffer.2015

    • Author(s)
      Md. Shahjahan, Hiroyuki Doi, Hironori Ando
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Synchronized expression of kisspeptin and its receptor genes in the diencephalon and pituitary of grass puffer during a lunar cycle.2015

    • Author(s)
      Md. Shahjahan,Hironori Ando
    • Organizer
      日本下垂体研究会第30回学術集会
    • Place of Presentation
      宇奈月国際会館セレネ(黒部)
    • Year and Date
      2015-08-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] クサフグの月周同調産卵回遊行動とホルモン2015

    • Author(s)
      安東宏徳
    • Organizer
      平成27年度日本水産学会春季大会シンポジウム「魚類行動生理学の基礎と水産研究への応用」
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 成長から成熟への転換機構:ベーリング海のサケで起きていること2015

    • Author(s)
      安東宏徳
    • Organizer
      形の科学研究センター2015シンポジウム
    • Place of Presentation
      新潟大学五十嵐キャンパス(新潟)
    • Year and Date
      2015-02-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 産卵回遊行動を調節する脳のしくみ‐月周産卵回遊魚クサフグをモデルとした研究2015

    • Author(s)
      安東宏徳
    • Organizer
      第1回新潟大学佐渡3 施設による森里海公開シンポジウム「佐渡から世界へ発信する最新の研究‐自然探求から、自然共生、自然再生へ」
    • Place of Presentation
      新潟大学理学部附属臨海実験所(佐渡)
    • Year and Date
      2015-02-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] 序論 回遊・渡り-巡(ホルモンから見た生命現象と進化シリーズ第6巻)2016

    • Author(s)
      浦野明央,安東宏徳
    • Total Pages
      169
    • Publisher
      裳華房
    • Related Report
      2016 Annual Research Report
  • [Book] サケとクサフグの産卵回遊 回遊・渡り-巡(ホルモンから見た生命現象と進化シリーズ第6巻)2016

    • Author(s)
      安東宏徳
    • Total Pages
      169
    • Publisher
      裳華房
    • Related Report
      2016 Annual Research Report
  • [Book] 光と脊椎動物の生活史(光と生命の事典)2015

    • Author(s)
      安東宏徳
    • Publisher
      朝倉書店
    • Related Report
      2014 Annual Research Report
  • [Remarks] http://www.sc.niigata-u.ac.jp/sc/sadomarine/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 新潟大学理学部附属臨海実験所

    • URL

      http://www.sc.niigata-u.ac.jp/sc/sadomarine/

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report

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Published: 2015-01-22   Modified: 2024-03-26  

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