亜鉛欠乏土壌におけるイネの生育を向上させる候補遺伝子の特定
| Project/Area Number |
14F04732
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Molecular biology
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| Research Institution | Japan International Research Center for Agricultural Sciences |
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Principal Investigator |
Wissuwa Matthias (WISSUWA Matthias) 国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 主任研究員 (90442722)
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| Co-Investigator(Kenkyū-buntansha) |
NANDA Amrit 国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 外国人特別研究員
NANDA AMRIT 国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 外国人特別研究員
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| Project Period (FY) |
2014-04-25 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2016: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2015: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2014: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | crown tissue / Zn deficiency / RNA sequencing / Zn efficient / Zn inefficient / gibberellin / 亜鉛欠乏 / 耐性メカニズム / 候補遺伝 / レトロトランスポゾンTos17 / イネ系統変異体 / 土壌の亜鉛欠乏 / 候補遺伝子同定 / 分子マーカーの開発 / 感受性イネ系統 / ゲノム / 遺伝子発現データ / マイクロアレーデータ |
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| Outline of Annual Research Achievements |
Studies were followed by a transcriptome analysis of crown tissue of genotypes contrasting for Zn deficiency tolerance. In this study she highlighted the molecular mechanisms triggered by early Zn deficiency in crown tissue, through RNA sequencing of two contrasting groups of several Zn efficient and Zn inefficient genotypes. This method allowed her to 1) identify several novel and well-known Zn transporters involved in Zn retranslocation from the crown to the shoot and roots in response to Zn deficiency; 2) determine that Zn deficiency triggers the conversion of soluble sugars into starch; and 3) detect several candidate genes possibly conferring Zn efficiency, including a monosaccharide transporter, a Zn finger domain-containing protein, a gibberellin stimulated family protein and a plasma membrane polypeptide family protein. This work has been submitted and is under review in the Journal of Experimental Botany:
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(5 results)
