| Project/Area Number |
14F04775
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
|
| Allocation Type | Single-year Grants |
|---|
| Section | 外国 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
SHIN JAE・WOO 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (60553849)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
LUGINBUEHL JOACHIM 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
LUGINBUEHL Joachim 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
|
|---|
| Project Period (FY) |
2014-04-25 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2016: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2015: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2014: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Induced neurons / cell reprogramming / single cell sequencing / gene network / Cell Reprogramming / Single cell sequencing / Gene network |
|---|
| Outline of Annual Research Achievements |
Single-cell RNA sequencing can disentangle highly diverse cell populations, and offers a way to unravel Transcriptional Regulatory Network (TRN) controlling cell-reprogramming. To this end, we generated a pool of 20 neuronal transcription factors (TFs) and sought to distinguish exogenous and endogenous TFs using single-cell RNAseq. Taking advantage of the dissimilarity of nucleotide sequences, we could identify exogenous and endogenous genes in induced neurons, and revealed a highly diverse subpopulation of induced neurons with defined gene signatures. We plan to perform validation experiments in collaboration with BSI/RIKEN. These results will be published within this fiscal year.
|
| Research Progress Status |
28年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
|
Report
(3 results)
Research Products
(3 results)
