| Project/Area Number |
14J03239
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
|
| Allocation Type | Single-year Grants |
|---|
| Section | 国内 |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
趙 艶春 京都大学, 生命科学研究科, 特別研究員(DC2)
|
|---|
| Project Period (FY) |
2014-04-25 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2015: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2014: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | Blood-thymus barrier / Endothelial cells / Claudin-5 |
|---|
| Outline of Annual Research Achievements |
(1) Analysis of the thymocyte number and T-cell development in Cld5 KO mice in abnormal conditions
Cld5 KO mice die soon after birth. In addition to the analysis of Cld5 KO mice in the first year of the research, I compared thymocyte phenotypes of Cld5 KO and control wild type fetuses after the exposure of LPS to examine whether BTB plays a role in protecting thymic microenvironment in an abnormal condition. I also injected dexamethasone, a kind of stress hormones, into the pregnant Cld5+/-× Cld5+/- mice with Cld5 KO fetuses, however, thymocyte development of both Cld5 KO and control mice were impaired by dexamethasone.
(2) Examine the effects of BTB disruption on T-cell selection using OT-I-TCR Tg Cld5 KO mice
Although I found that intravenous injection of ova-peptide (257-264) into pregnant mice could affect the thymocyte development of OT-I-TCR Tg fetuses, it is hard to collect enough OT-I-TCR Cld5 KO fetuses to get stable and reliable conclusion. Thus, this assay system was not suitable to examine the effects of BTB disruption.
(3) Phenotypic analysis of thymic vascular endothelial cells (VECs) by flow cytometry
After establishing the method to examine Cld5 expression by FACS, I found that the proportion of Cld5+ thymic VECs decreases with age. To understand the differences of Cld5+ and Cld5- VECs, I co-stained Cld5 with various vascular endothelial markers and found that the expression of Ly6c is well correlated to Cld5 expression on thymic VECs, although its physiological significance remains to be verified.
|
| Research Progress Status |
27年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
27年度が最終年度であるため、記入しない。
|
Report
(2 results)
Research Products
(1 results)
