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光分解型3次元培養基材を用いたテーラーメード抗がん剤感受性検査法の開発

Research Project

Project/Area Number 14J07186
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

田村 磨聖  国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 特別研究員(PD)

Project Period (FY) 2014-04-25 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords細胞分離 / がん / 細胞形態 / テーラーメード検査 / 抗がん剤薬剤感受性試験 / 光分解性ゲル / クリック反応 / 3次元培養 / 3次元細胞培養 / 感受性試験
Outline of Annual Research Achievements

本テーマの目的は、腫瘍組織由来の細胞群から薬剤耐性の細胞あるいは悪性がん細胞(浸潤細胞)を選び出し、テーラーメード検査に用いる細胞材料を提供することである。
H26年度は、本テーラーメード検査用の光分解型培養基材の合成を行い、細胞分離が可能な培養基材を開発し、H27年度には、クリック反応による光分解型ゲルの合成を行うことで、生理活性物質を添加できるとともに、細胞の浸潤挙動をより観察しやすいゲルを開発した。本年度は、化学発がんマウス腫瘍由来の細胞を用いた検討を実施するとともに、前年度まで改良してきたゲルを用いてマウス乳がん由来細胞の薬剤感受性試験、あるいは細胞の形態とがん細胞悪性度の関係を検討した。
マウス乳がん由来細胞を光分解型ゲル中で培養し、増殖した細胞の増殖形態を基に、顆粒状細胞とコロニー状細胞の2種類の細胞に分類した。光照射により標的細胞周囲のゲルを分解し、目的とする2種類の細胞を得て実験に用いる細胞を樹立した。このとき、種類ごとに2回同様の作業を行い、細胞分離を行わない母株も併せて合計5種の細胞株を実験に用いた。これらの分離細胞を用いて、光分解性ゲル中の細胞形態とがん悪性度に関して検討を行った。光分解性ゲル中で顆粒状に増殖した細胞はスフェロイドを形成せず、コロニー状に増殖した細胞はスフェロイドを形成した。また、これらの細胞をヌードマウスに移植すると、コロニー状の細胞は皮下で腫瘍を増大する性質が強く、顆粒状の細胞は移植部から他の臓器へと転移し易い性質を示すことが分かった。一方で、薬剤感受性と細胞の形態についても、薬剤の種類によっては関連し得るデータを得た。
更なるメカニズムの解析、細胞の形態と薬剤感受性あるいはその背景となる遺伝子発現・変異の検討などは今後の研究課題として残った。

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (21 results)

All 2017 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (13 results) (of which Int'l Joint Research: 4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Radiotherapy for cancer using X-ray fluorescence emitted from iodine2017

    • Author(s)
      Masato Tamura, Hiromu Ito, Hirofumi Matsui
    • Journal Title

      Scientific reports

      Volume: 7 Issue: 1 Pages: 43667-43667

    • DOI

      10.1038/srep43667

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment2015

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori
    • Journal Title

      Scientific reports

      Volume: 5 Issue: 1 Pages: 15060-15060

    • DOI

      10.1038/srep15060

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Synthesis, Photophysical Properties, and Biological Evaluation of trans-Bisthioglycosylated Tetrakis(fluorophenyl)chlorin for Photodynamic Therapy2015

    • Author(s)
      S. Hirohara, C. Oka, M. Totani, M. Obata, J. Yuasa, H. Ito, M. Tamura, H. Matsui, K. Kakiuchi, T. Kawai, M. Kawaichi, M. Tanihara
    • Journal Title

      J. Med. Chem.

      Volume: 58 Issue: 21 Pages: 8658-8670

    • DOI

      10.1021/acs.jmedchem.5b01262

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Micropatterning of click-crosslinked and photodegradable hydrogels for 3D perfusion culture2015

    • Author(s)
      Fumiki Yanagawa, Masato Tamura, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori,
    • Journal Title

      Proceeding of MicroTAS

      Volume: 1 Pages: 615-617

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A mitochondrial superoxide theory for oxidative stress diseases and aging2015

    • Author(s)
      Hiroko P. Indo, Hsiu-chuan Yen, Ikuo Nakanishi, Ken-ichiro Matsumoto, Masato Tamura, Yumiko Nagano, Hirofumi Matsui, Oleg Gusev, Richard Cornette, Takashi Okuda, Yukiko Minamiyama, Hiroshi Ichikawa, Shigeaki Suenaga, Misato Oki, Tsuyoshi Sato, Toshihiko Ozawa, Daret K. St. Clair, Hideyuki J. Majima
    • Journal Title

      Journal of clinical biochemistry and nutrition

      Volume: 56 Pages: 1-7

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques2014

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Hirofumi Matsui, Toshiyuki Kanamori
    • Journal Title

      Scientific reports

      Volume: 4 Issue: 1 Pages: 4793-4793

    • DOI

      10.1038/srep04793

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorescence-based co-culture of normal and cancerous cells as an indicator of therapeutic effects in cancer2014

    • Author(s)
      Masato Tamura, Hirofumi Matsui, Ichinosuke Hyodo, Junko Tanaka, Yoshihiro Miwa
    • Journal Title

      European journal of pharmaceutical sciences

      Volume: 63C Pages: 1-7

    • DOI

      10.1016/j.ejps.2014.06.014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 細胞形態を指標とした光分解性ゲルからの細胞分離技術の開発2017

    • Author(s)
      田村磨聖、渋田真結、杉浦慎治、加藤竜司、柳沢真澄、松井裕史、蟹江慧、佐藤琢、 高木俊之、須丸公雄、金森敏幸
    • Organizer
      つくば医工連携フォーラム2017
    • Place of Presentation
      物質・材料研究機構 (茨城)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Click-crosslinkable and photodegradable gelatin hydrogels for cell separation2016

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori
    • Organizer
      ICBS2016
    • Place of Presentation
      Ito Hall at the University of Tokyo (Tokyo)
    • Year and Date
      2016-11-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光照射による形態別細胞分離システムの開発2016

    • Author(s)
      田村磨聖、渋田真結、杉浦慎治、加藤竜司、柳沢真澄、松井裕史、蟹江慧、佐藤琢、高木俊之、須丸公雄、金森敏幸
    • Organizer
      Cheminas 34th
    • Place of Presentation
      幕張メッセ国際会議場 (千葉)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of photodegradable gelatin hydrogels and image analysis technique for automatic optical cell separation system based on the cellular morphology in embedding culture2016

    • Author(s)
      Masato Tamura, Shinji Sugiura, Taku Satoh, Toshiyuki Kanamori, Mayu Shibuta, Kei Kanie, Ryuji Kato, Hirofumi Matsui, Masumi Yanagisawa
    • Organizer
      10th World Biomaterials Congress (WBC) 2016
    • Place of Presentation
      Montreal Convention Center (Montereal, Canada)
    • Year and Date
      2016-05-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of an optical cell separation based on a cellular shape using click-crosslinkable and photodegradable gelatin hydrogels2016

    • Author(s)
      Masato Tamura, Shinji Sugiura, Taku Satoh, Toshiyuki Takagi, Toshiyuki Kanamori, Mayu Shibuta, Kei Kanie, Ryuji Kato, Hirofumi Matsui, Masumi Yanagisawa
    • Organizer
      The 8th international conference on Microtechnologies in medicine and Biology MMB 2016
    • Place of Presentation
      Seoul National University (Seoul, Korea)
    • Year and Date
      2016-04-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光分解性ゲル包埋培養からの形態別細胞分離システムの開発2016

    • Author(s)
      田村 磨聖、渋田真結、杉浦慎治、加藤竜司、柳沢真澄、松井裕史、蟹江慧、佐藤琢、 高木俊之、須丸公雄、金森敏幸
    • Organizer
      第23回HAB研究機構学術年会
    • Place of Presentation
      産業技術総合研究所 (茨城)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 形状によるがん細胞選別システムの開発2015

    • Author(s)
      田村磨聖
    • Organizer
      32nd Cheminas
    • Place of Presentation
      北九州国際会議場(福岡)
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Click-crosslinkable and photodegradable gelatin hydrogels for threedimensional microscale cell processing2015

    • Author(s)
      Masato Tamura
    • Organizer
      7th international symposium on microchemistry and microsystems
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2015-06-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光分解性ゲルを用いた3次元培養細胞の選択的分離2015

    • Author(s)
      田村磨聖, 柳川史樹, 杉浦慎治, 高木俊之, 須丸公雄, 松井裕史, 金森敏幸
    • Organizer
      細胞アッセイ技術の現状と将来
    • Place of Presentation
      東京
    • Year and Date
      2015-01-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Optical cell separation from 3D culture environment in photodegradable hydrogels for pure culture techniques2014

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Hirofumi Matsui, Toshiyuki Kanamori
    • Organizer
      EMBS Micro and Nanotechnology in Medicine conference
    • Place of Presentation
      hawaii
    • Year and Date
      2014-12-07 – 2014-12-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Photodegradable hydrogels for optical cell separation from three-dimensional environment2014

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Hirofumi Matsui, Toshiyuki Kanamori
    • Organizer
      IPC 2014
    • Place of Presentation
      Ibaraki
    • Year and Date
      2014-12-02 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Optical cell separation with photodegradable hydrogels rom three-dimensional environment or pure culture techniques2014

    • Author(s)
      Masato Tamura, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Takagi, Kimio Sumaru, Hirofumi Matsui, Toshiyuki Kanamori
    • Organizer
      PN&G 2014
    • Place of Presentation
      Tokyo
    • Year and Date
      2014-11-10 – 2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光分解性ゲルを用いた局所光照射による3次元培養細胞の選択的抽出2014

    • Author(s)
      田村磨聖, 柳川史樹, 杉浦慎治, 高木俊之, 須丸公雄, 松井裕史, 金森敏幸
    • Organizer
      第21回HAB研究機構学術年会
    • Place of Presentation
      東京
    • Year and Date
      2014-05-16 – 2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] サンドイッチ培養用培養容器2015

    • Inventor(s)
      杉浦慎治、佐藤琢、田村磨聖、金森敏幸、柳沢真澄
    • Industrial Property Rights Holder
      産業技術総合研究所、エンジニアリングシステム株式会社
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-065009
    • Filing Date
      2015-03-26
    • Related Report
      2014 Annual Research Report

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Published: 2015-01-22   Modified: 2024-03-26  

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