Functional analysis of the C. elegansgenome by a systematic gene knock-down
| Project/Area Number |
15011261
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | RIKEN |
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Principal Investigator |
SUGIMOTO Asako RIKEN, Laboratory for Developmental Genomics, Team Leader, 発生ゲノミクス研究チーム, チームリーダー (80281715)
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| Co-Investigator(Kenkyū-buntansha) |
MITANI Shohei Tokyo Women's Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (90192757)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥24,000,000 (Direct Cost: ¥24,000,000)
Fiscal Year 2004: ¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2003: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Keywords | nematode / C. elegans / RNAi / deletion mutant / functional genomics / C. elegans |
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| Research Abstract |
In this study, we aimed to understand the framework of genetic cascades and networks that regulate the development and behavior of multicellular organisms, using the nematode C. elegans as a model system. To this end, we performed systematic analyses of loss-of-function phenotypes caused by either RNAi or deletion mutations.
1. A general phenotype analysis was performed for〜6000 genes by an RNAi-by-soaking method, and 30% of them were shown to be essential for developmental processes. We further performed embryonic phenotype profiling of the 833 embryonic lethal genes identified in this screen. A cluster analysis of the phenotypic profiles successfully grouped the gene sets that were known to function in the same developmental/biological processes. Thus, this method will be useful to predict gene functions of uncharacterized genes. We compiled these data and established "A C. elegans RNAi phenome database" accessible via Web. We also performed the post-embryonic functions of the embryonic lethal genes by a developmental stage-specific RNAi method.
2. Systematic screening for deletion mutants of transcription factors in C. elegans was conducted. We have isolated mutants corresponding to 437 genes, which are more than two thirds of whole transcription factors. We are also isolating many other mutants and simultaneously use them to reveal gene function. Among the mutants, we found that ags-3 mutants show abnormal chemosensory perception, body size and moving activity. We found that ags-3 phenotypes are suppressed by an egl-4 (PKG) mutant. AGS-3 is known to play an important role in cocaine addiction. We found that AGS-3 and PKG from both nematode and mouse interact with each other.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways.2006
Author(s)
Duchaine T, Wohlshlegel JA, Kennedy S, Bei Y, Conte D, Pang K, Brownell DR, Harding S, Mitani S, Ruvkun G, Yates IIIJr, Mello C
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Journal Title
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways.2006
Author(s)
Duchaine TF, Wohlshlegel JA, Kennedy S, Bei Y, Conte D, Pang K, Brownell DR, Harding S, Mitani S, Ruvkun G, Yates III JR, Mello CC
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development.2005
Author(s)
Yanagi, K., Mizuno, T., Tsuyama, T., Tada, S., Iida, Y., Sugimoto, A., Eki, T., Enomoto, T., Hanaoka, F.
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Journal Title
J Biol Chem 280
Pages: 19689-19694
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Essential role of the C.elegans Arp2/3 complex in cell migration during ventral enclosure.2003
Author(s)
Saws, M., Suetsugu, S., Sugimoto, A., Miki, H., Yamamoto, M., Takenawa, T.
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Journal Title
J Cell Sci 116
Pages: 1505-1518
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Involvement of chondroitin proteoglycans in cytokinesis of embryonic cells of the nematode Caenorhabditis elegans.2003
Author(s)
Mizuguchi, S., Uyama, T., Kitagawa, H., Nomura, K.H., Dejima, K., Gengyo-Ando, K., Mitani, S., Sugahara, K., Nomura, K.
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Journal Title
Nature 423
Pages: 443-448
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Cell Corpse Engulfment Mediated by C.elegans Phosphatidylserine Receptor Through CED-5 and CED-12.2003
Author(s)
Wang, X., Wu, Y -C., Fadok, VA., Lee, M-C., Gengyo-Ando, K., Cheng, L-C., Ledwich, D., Hsu, P-K., Chen, J-Y., Chou, B-K., Henson, P., Mitani.S., Xue, D.
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Journal Title
Science 302
Pages: 1563-1566
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Essential role of the C. elegans Arp2/3 complex in cell migration during ventral enclosure.2003
Author(s)
Sawa, M., Suetsugu, S., Sugimoto, A., Miki, H., Yamamoto, M., Takenawa, T.
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Journal Title
J Cell Sci 116
Pages: 1505-1518
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Cell Corpse Engulfment Mediated by C. elegans Phosphatidylserine Receptor Through CED-5 and CED-12.2003
Author(s)
Wang, X., Wu, Y-C., Fadok, VA., Lee, M-C., Gengyo-Ando, K., Cheng, L-C., Ledwich, D., Hsu, P-K., Chen, J-Y., Chou, B-K., Henson, P., Mitani, S., Xue, D.
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Journal Title
Science 302
Pages: 1563-1566
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Publications] Sawa, M., Suetsugu, S., Sugimoto, A, Miki, H., Yamamoto, M., Takenawa, T.: "Essential role of the C.elegans Arp2/3 complex in cell migration during ventral enclosure."J Cell Sci. 116. 1505-1518 (2003)
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[Publications] Wang X., Wu Y-C., Fadok VA., Lee M-C., Gengyo-Ando K., Cheng L-C., Ledwich D., Hsu P-K., Chen J-Y., Chou B-K., Henson P., Mitani S., Xue D.: "Cell Corpse Engulfment Mediated by C.elegans Phosphatidylserine Receptor Through CED-5 and CED-12."Science. 302. 1563-1566 (2003)
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