生殖細胞の発生プロセス・再プログラム化とエピジェネティクス

• Project/Area Number: 15080101
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: 中辻 憲夫 Kyoto University, 物質-細胞統合システム拠点, 教授 (80237312)
• Co-Investigator(Kenkyū-buntansha): 小倉 淳郎 理化学研究所, バイオリソースセンター, 室長 (20194524) ; 佐々木 裕之 国立遺伝学研究所, 総合遺伝研究系, 教授 (30183825) ; 塩田 邦郎 東京大学, 大学院・農学生命科学研究科, 教授 (80196352) ; 仲野 徹 大阪大学, 大学院・生命機能研究科, 教授 (00172370) ; 松居 靖久 (松井 靖久) 東北大学, 加齢医学研究所, 教授 (40241575)
• Project Period (FY): 2003 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥53,700,000 (Direct Cost: ¥53,700,000); Fiscal Year 2008: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2007: ¥8,200,000 (Direct Cost: ¥8,200,000); Fiscal Year 2006: ¥8,500,000 (Direct Cost: ¥8,500,000); Fiscal Year 2005: ¥17,000,000 (Direct Cost: ¥17,000,000); Fiscal Year 2004: ¥8,500,000 (Direct Cost: ¥8,500,000); Fiscal Year 2003: ¥8,500,000 (Direct Cost: ¥8,500,000)
• Keywords: 発生・分化 / 遺伝学 / 遺伝子 / 動物 / バイオテクノロジー / 生殖細胞 / 再プログラム化 / エピジェネティクス / 哺乳類 / ゲノムインプリンティング / クローン動物 / DNAメチル化 / 発生生物学

Research Abstract

当該特定領域研究は、発生生物学、実験動物学、遺伝学、分子生物学など多分野にまたがる学際的研究グループを形成しており、多様なバックグラウンドをもつ研究者による研究課題を推進してきた。平成19年度までに、極めて多くの研究成果が得られて優れた学術論文として発表されている。それと同時に、多様な組み合わせで各研究者の得意分野によるシナジーを生み出しながら、多くの共同研究が行われ成功してきた。平成20年度は、本総括班の活動の総仕上げとして、これら特定領域研究によって過去5年間に生み出されて研究成果のとりまとめを行うと同時に、研究成果報告書を作成した。なお、研究成果報告書の体裁に従って研究成果発表の詳細なリストなど報告資料として作成した報告書に加えて、研究成果を広く研究者コミュニティーに対する広報活動として周知させることを目的として、研究成果を読みやすい体裁で取りまとめた報告書も並行して作成し配布した。[連携研究者]
独立行政法人理化学研究所・バイオリソースセンター 小倉淳郎 領域事務担当者としての連絡調整
国立遺伝学研究所・総合遺伝研究系 佐々木裕之 ゲノム刷り込み研究の企画調整
東京大学・農学生命科学研究科 塩田邦郎 エピジェネティクス研究の企画調整
大阪大学・大学院生命機能研究科 仲野徹 生殖細胞特性研究の企画調整
東北大学・加齢医学研究所 松居靖久 生殖細胞発生研究の企画調整

Research Products

• [Journal Article] In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells (2009)
  • Author(s): Yamauchi K
  • Journal Title: PLoS ONE
  • Peer Reviewed
• [Journal Article] Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline : Germinal granules inmammals (2008)
  • Author(s): Chuma S
  • Journal Title: Mol Cell Endocrinol
  • Peer Reviewed
• [Journal Article] Homing of mouse spermatogonial stem cells to germline nichehe pends on betal-integrin (2008)
  • Author(s): Kanatsu-Shinohara M
  • Journal Title: Cell Stem Cell 3 Pages: 533-54
  • Peer Reviewed
• [Journal Article] Long-Term Culture of Male Germline Stem Cells from Hamster Testes. (2008)
  • Author(s): Kanatsu-Shinohara, et. al.
  • Journal Title: Biol Reprod 78 Pages: 611-617
  • Peer Reviewed
• [Journal Article] Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells. (2007)
  • Author(s): Honda, et. al.
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 12389-12394
  • Peer Reviewed
• [Journal Article] The Developmental Abi1ity of Vitrified Oocytes from Different Mouse Strains Assessed by Parthenogenetic Activation and Intracytoplasmic Sperm Injection. (2007)
  • Author(s): Endoh, et. al.
  • Journal Title: J Reprod Dev 53 Pages: 1199-1206
  • Peer Reviewed
• [Journal Article] Tudor-related Proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP : domain composition, intracellular localization, and function in male germ cells in mice. (2007)
  • Author(s): Hosokawa, M.
  • Journal Title: Dev.Biol. 301 Pages: 38-52
• [Journal Article] Targeted chromosome elimination from ES-somatic hybrid cells. (2007)
  • Author(s): Matsumura, H.
  • Journal Title: Nature Methods 4 Pages: 23-25
• [Journal Article] Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. (2007)
  • Author(s): Shinmen, A.
  • Journal Title: Mol.Reprod.Dev. (in press)
• [Journal Article] Tdrd1/Mtr-1, a tudor-related gene, is essential for male germ-cell differentiation and nuage/germinal granule formation in mice. (2006)
  • Author(s): Chuma, S.
  • Journal Title: Proc.Natl.Acad.Sci.USA 103 Pages: 15894-15899
• [Journal Article] Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination. (2006)
  • Author(s): Shinohara, T.
  • Journal Title: Proc.Natl.Acad.Sci.USA 103 Pages: 13624-13628
• [Journal Article] Improvement of cumulus-free oocyte maturation in vitro and its application to microinsemination with primary spermatocytes in mice. (2006)
  • Author(s): Miki, H.
  • Journal Title: J.Reprod.Dev. 52 Pages: 239-248
• [Journal Article] Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer. (2006)
  • Author(s): lnoue, K.
  • Journal Title: J.Cell Sci. 119 Pages: 1985-1991
• [Journal Article] Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring. (2006)
  • Author(s): Ogonuki, N.
  • Journal Title: Proc.Natl.Acad.Sci.USA 103 Pages: 13098-13103
• [Journal Article] Intra-strain polymorphisms are detected but no genomic alteration is found in cloned mice. (2006)
  • Author(s): Gotoh, K.
  • Journal Title: Biochem.Biophys.Res.Commun. 348 Pages: 166-169
• [Journal Article] Chorioallantoic placenta defects in cloned mice. (2006)
  • Author(s): Wakisaka, N.
  • Journal Title: Biochem.Biophys.Res.Commun. 349 Pages: 106-114
• [Journal Article] A histone H3 methyltransferase controls epigenetic events required for meiotic prophase. (2005)
  • Author(s): Hayashi, K.
  • Journal Title: Nature 438(7066) Pages: 374-378
• [Journal Article] RNF17, a component of the mammalian germ cell nuage, is essential for spermiogenesis. (2005)
  • Author(s): Pan, J.
  • Journal Title: Development 132(18) Pages: 4029-4039
• [Journal Article] Genetic and epigenetic properties of mouse male germline stem cells during long-term culture. (2005)
  • Author(s): Kanatsu-Shinohara, M.
  • Journal Title: Development 132(18) Pages: 4155-4163
• [Journal Article] Microinsemination and nuclear transfer using male germ cells. (2005)
  • Author(s): Ogura, A.
  • Journal Title: Int.Rev.Cytol. 246 Pages: 189-229
• [Journal Article] Extensive and orderly reprogramming of genome-wide chromatin modification associated with specification and early development of germ cells in mice. (2005)
  • Author(s): Seki, Y.
  • Journal Title: Dev.Biol. 278(2) Pages: 440-458
• [Journal Article] Birth of mice produced by germ cell nuclear transfer. (2005)
  • Author(s): Miki, H.
  • Journal Title: Genesis 41(2) Pages: 81-86
• [Journal Article] Nanog expression in mouse germ cell development. (2005)
  • Author(s): Yamaguchi, S.
  • Journal Title: Gene Expr.Patterns 5(5) Pages: 639-646
• [Journal Article] Pluripotential competence of cells associated with Nanog activity. (2005)
  • Author(s): Hatano, S.Y.
  • Journal Title: Mech.Dev. 122(1) Pages: 67-79
• [Journal Article] Generation of cloned mice by direct nuclear transfer from natural killer T cells. (2005)
  • Author(s): Inoue, K.
  • Journal Title: Curr.Biol. 15(12) Pages: 1114-1118
• [Journal Article] Dynamic rearrangement of telomeres during spermatogenesis in mice. (2005)
  • Author(s): Tanemura, K.
  • Journal Title: Dev.Biol. 281(2) Pages: 196-207
• [Journal Article] Genomic imprinting of XX spermatogonia and XX oocytes recovered from XX/XY chimeric testis. (2005)
  • Author(s): Isotani, A.
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 4039-4044
• [Journal Article] Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis. (2005)
  • Author(s): Chuma, S.
  • Journal Title: Development 132 Pages: 117-122
• [Journal Article] Birth of offspring after transfer of Mongolian gerbil (Meriones unguiculatus) embryos cryopreserved by vitrification. (2005)
  • Author(s): Mochida, K.
  • Journal Title: Molecular Reproduction and Development 70 Pages: 464-470
• [Journal Article] Birth of Mice Produced by Germ Cell Nuclear Transfer. (2005)
  • Author(s): Miki, H.
  • Journal Title: Genesis 41 Pages: 81-86
• [Journal Article] Cytoplasmic Asters are Required for Progression past the First Cell Cycle in Cloned Mouse Embryos. (2004)
  • Author(s): Miki, H.
  • Journal Title: Biology of Reproduction 71 Pages: 2022-2028
• [Journal Article] Generation of pluripotent stem cells from neonatal mouse testis. (2004)
  • Author(s): Kanatsu-Shinohara, M.
  • Journal Title: Cell 119 Pages: 1001-1012
• [Journal Article] RNA interference during spermatogenesis in mice.
  • Author(s): Shoji, M.
  • Journal Title: Dev.Biol. (印刷中)
• [Journal Article] Long-term culture of mouse male germline stem cells under serum- or feeder-free conditions.
  • Author(s): Kanatsu-Shinohara, M.
  • Journal Title: Biology of Reproduction (印刷中)
• [Presentation] Characterization of thecal stem cells and primordial oocytes concomitantly isolated from mouse neonatal ovaries (2007)
  • Author(s): Ogura, A.
  • Organizer: The 4th Annual meeting of the Asian Reproductive Biotechnology Society
  • Place of Presentation: Singapore
• [Book] 生殖細胞の発生・エピジェネティクスと再プログラム化(蛋白質核酸酵素増刊) (2007)
  • Author(s): 小倉淳郎, 他(編集)
  • Total Pages: 206
  • Publisher: 共立出版
• [Book] クロマチン・染色体実験プロトコール (2004)
  • Author(s): 井上貴美子
  • Total Pages: 231
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/rc01/tokutei/index-t.html
• [Publications] Kasai, S., Chuma, S., Motoyama, N., Nakatsuji, N.: "Haploinsufficiency of Bcl-x leads to male specific defects in fetal germ cells : differential regulation of germ cell apoptosis between the sexes."Dev.Biol.. 264. 202-216 (2003)
• [Publications] Chuma, S., Hiyoshi, M., Yamamoto, A., Hosokawa, M., Nakatsuji, N.: "Mouse Tudor Repeat-1 (MTR-1) is a novel component of chromatoid bodies/nuages in male germ cells and forms a complex with snRNPs."Mech.Dev.. 120. 979-990 (2003)
• [Publications] Ogura, A., Ogonuki, N., Inoue, K., Mochida, K.: "New microinsemination techniques for laboratory animals."Theriogenology. 59. 87-94 (2003)
• [Publications] Ogonuki, N., Tsuchiya, H., Hirose, Y., Okada, H., Ogura, A., Sankai, T.: "Pregnancy by the tubal transfer of embryos developed after injection of round spermatids into oocyte cytoplasm of the cynomolgus monkey (Macaca fascicularis)."Hum.Reprod.. 18. 1273-1280 (2003)
• [Publications] Ogonuki, N., Mochida, K., Inoue, K., Matsuda, J., Yamamoto, Y., Takano, K., Ogura, A.: "Fertilization of Oocytes and Birth of Normal Pups Following intracytoplasmic Injection with Spermatids in Mastomys (Praomys coucha)."Biol.Reprod.. 68. 1821-1827 (2003)
• [Publications] Kanatsu-Shinobhara, M., Ogonuki, N., Inoue, K., Ogura, A., Toyokuni, S., Shinohara, T.: "Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stem cells."Hum.Reprod.. 18. 2660-2667 (2003)
• [Publications] Kanatsu-Shinohara, M., Ogonuki, N., Inoue, K., Ogura, A., Toyokuni, K., Kogishi, T., Honjo, T., Shinohara, T.: "Allogeneic offspring produced by male germ line stem cell transplantation into infertile mouse testis."Biol.Reprod.. 68. 167-173 (2003)
• [Publications] Kanatsu-Shinohara, M., Ogonuki, N., Inoue, K., Miki, H., Ogura, A., Toyokuni, S., Shinohara, T.: "Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells."Biol.Reprod.. 69. 612-616 (2003)
• [Publications] Inoue, K, Ogonuki, N., Mochida, K., Yamamoto, Y., Takano, K., Kohda, T., Ishino, F., Ogura, A.: "Effects of Donor Cell Type and Genotype on the Efficiency of Mouse Somatic Cell Cloning."Biol.Reprod.. 69. 1394-1400 (2003)
• [Publications] Nakamura, T., Yao, R., Ogawa, T., Suzuki, T., Ito, C., Tsunekawa, N., Inoue, K., Ajima R., Miyasaka, T., Yoshida, Y., Ogura, A., Toshimori, K., Nose, T., Yamamoto, T., Noda, T.: "Oligo-astheno-teratozoospermia in mice lacking CCR4-associated factor 1, a novel regulator of RXRb."Nat.Genet.. (in press). (2004)
• [Publications] Miki, H., Lee, J., Inoue, K., Ogonuki, N., Noguchi, Y., Mochida, K., Kohda, T., Nagashima, H., Ishino, F., Ogura, A.: "Microinsemination with first-wave round spermatids from immature male mice."J.Reprod.Dev.. 50. 131-137 (2004)
• [Publications] Kai, M., Irie, M., Okutsu, T., Inoue, K., Ogonuki, N., Miki, H., Yokoyama, M., Migishima, R., Muguruma, K., Fujimura, H., Kohda, T., Ogura, A., Kaneko-Ishino, T., Ishion, F.: "The Novel Dominant Mutation Dspd Leads to a Severe Spermiogenesis Defect in Mice."Biol.Reprod.. (in press). (2004)
• [Publications] Inoue, K., Ogonuki, N., Yamamoto, Y., Takano, K., Miki, H., Mochida, K., Ogura, A.: "Tissue-specific distribution of donor mitochondrial DNA in cloned mice produced by somatic cell nuclear transfer."Genesis. (in press). (2004)
• [Publications] Fulka-Jr, J., Miyashita, N., Nagai, T., Ogura, A.: "Do cloned mammals skip a reprogramming step?"Nat.Biotechnol.. 22. 25-26 (2004)
• [Publications] 中辻憲夫, 中馬新一郎: "多能性幹細胞と胚形成・生殖系列「再生医学の基礎 幹細胞と臓器形成」(中辻憲夫編)"名古屋大学出版会. 29 (2003)