| Project/Area Number |
15200038
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
MATSUDA Takehisa KYUSHU UNIVERSITY, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (60142189)
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| Co-Investigator(Kenkyū-buntansha) |
KIDOAKI Satoru KYUSHU UNIVERSITY, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (10336018)
SHIMOKAWA Hiroaki KYUSHU UNIVERSITY, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (00235681)
内海 裕一 姫路工業大学, 高度産業科学技術研究所, 助教授 (80326298)
服部 正 姫路工業大学, 高度産業科学技術研究所, 教授 (70326297)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000)
Fiscal Year 2005: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2004: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2003: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
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| Keywords | stent / scaffold / endothelial cell / 内皮前駆細胞 / 光化学 |
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| Research Abstract |
This study aimed at developing high biocompatible stent technology based on ex vivo cell seeding by combination of cell engineering, matrix engineering and scaffold engineering, by which rapid complete endothelialization on an implanted stent as to fully prevent thrombus formation, thus avoiding stenosis and occlusion, will be achieved. To this end, the authors developed endothelial cell and endothelial progenitor cell, both of which are harvested from autologous tissue or blood, are seeded and cultured to form monolayered tissue on the whole body of stent. As for matrix engineering, vinylated biomolecules include albumin, gelatin and heparin were photopolymerized under visible light-irradiation to form completely cover the stent surface prior to cell seeding. As for scaffold engineering, the authors devised electrospinning method using synthetic biodegradable elastomer (copolymer of lactide and caprolactone) to form a mesh-type cover stent. Bioactive substances such as collagen, heparin and growth factor were also co-electronspun. It is envisaged that the combination of these technologies expectantly enhances the thrombus-free condition which starts immediately after implantation.
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