Studies on vertebrate developmental genes by transposon-mediated gene trapping
| Project/Area Number |
15201041
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
基礎ゲノム科学
|
|---|
| Research Institution | National Institute of Genetics. |
|---|
Principal Investigator |
KAWAKAMI Koichi National Institute of Genetics, Department of Developmental Genetics, Associate professor, 個体遺伝研究系, 助教授 (70195048)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥35,620,000 (Direct Cost: ¥27,400,000、Indirect Cost: ¥8,220,000)
Fiscal Year 2005: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2004: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2003: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
|
|---|
| Keywords | Molecular Biology / Developmental Genetics / Genome Biology / Zebrafish / Transposon / Transgenic fish / Gene expression / Gene trapping / エンハンサートラップ / インサーショナルミュータジェネシス / 器官形成 / wntシグナル / 挿入変異 / 転移酵素 / トランスジェニック動物 |
|---|
| Research Abstract |
(1)We injected a transposon-donor plasmid containing a To12 construct and mRNA encoding the transposase into zebrafish fertilized eggs and demonstrated that the transposon construct can be integrated in the genome of the germ cells very efficiently. This method is now widely used as a standard method to create transgenic fish.
(2)We constructed the To12 construct containing a splice acceptor and the GFP reporter gene and created random integration of this construct in the zebrafish genome. When the splice acceptor trapped a transcript of an endogenous gene, the GFP gene is expressed in a pattern similar to that of the endogenous gene. This was the first successful demonstration of gene trapping in zebrafish.
(3)We have been making efforts to distribute this useful methodology by publication of manuscripts, talks in international meetings, writing review articles, a training course and collaboration.
(4)In the course of the transposon studies in zebrafish, we also found that the To12 transposon system is also active in mouse and Xenopus. To12 will be a useful gene transfer vector in these model vertebrates.
|
Report
(4 results)
Research Products
(30 results)
-
-
-
-
-
-
[Journal Article] The zebrafish pob gene encodes a novel protein required for survival of red cone photoreceptor cells2005
Author(s)
Taylor, M.R., Kikkawa, S., Diez-Juan, A., Ramamurthy, V., Kawakami, K., Carmeliet, P., Brockerhoff, S.E.
-
Journal Title
Genetics 170
Pages: 263-273
Related Report
-
[Journal Article] The Zebrafish pob gene encodes a novel protein required for survival of red cone photoreceptor cells2005
Author(s)
Taylor, M.R., Kikkawa, S., Diez-Juan, A., Ramamurthy, V., Kawakami, K., Carmeliet, P., Brockerhoff, S.E.
-
Journal Title
Genetics Feb.16(on line publication)
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish2004
Author(s)
Kawakami, K., Takeda, H., Kawakami, N., Kobayashi, M., Matsuda, N., Mishina, M.
-
Journal Title
Developmental Cell 7
Pages: 133-144
Related Report
-
-
-
-
-
-
-
-
-
-
[Publications] Clark, M.S., Edwards, Y.J.K., Peterson, D., Clifton, S.W., Thompson, A.J., Sasaki, M., Suzuki, Y., Kikuchi, K., Watabe, S., Kawakami, K., Sugano, S., Elgar, G., Johnson, S.L.: "Fugu ESTs : New resources for transcription analysis and genome annotation."Genome Research. 13. 2747-2753 (2003)
-
