The analysis of ERECTA gene, which is responsible for the elongation of the stem, in Arabidopsis.

• Project/Area Number: 15207001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: KOMEDA Yoshibumi The University of Tokyo, Graduate School of Science, Professor, 大学院理学系研究科, 教授 (10124215)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Mitsuhiro The University of Tokyo, Graduate School of Science, Research Fellow, 大学院理学系研究科, 助手 (40361563)
• Project Period (FY): 2003 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥48,230,000 (Direct Cost: ¥37,100,000、Indirect Cost: ¥11,130,000); Fiscal Year 2006: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2005: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2004: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2003: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
• Keywords: Arabidopsis / stem / elongation / erecta mutants / floral stem / 花序形態 / 茎伸長 / ERECTA遺伝子 / ERBP遺伝子 / 頂端分裂組織

Research Abstract

The aim of this study is: to understand the processes during the development of higher plants especially for the elongation of the floral stems in Arabidopsis. For that, our tools are to isolate mutants and to analyze the responsible genes.
1.The study of two genes for erecta-binding proteins.
The erbpl and 2 double mutants expressed seedling-lethality. The ontogenic defective point was searched by observation of the embryos from globular stage to maturity. The defect revealed after the onset of the heart-stage-embryo. The relation to auxin was determined by using auxin-reporter DR5-uidA.
2.To search new factors for the regulation of ERECTA gene.
We collected many mutants which have reduced expression of ER gene by using ER-uidA(from E. coli) reporter. We isolated mutants carrying reduced expression of erecta (as rex) 1 and 2 genes. Their responsible genes encode for pectin-synthesis and for factors for cellar organella, respectively. The regulation of related genes were determined under the rexl and 2 background to know the regulation.

Research Products

• [Journal Article] The dwarf phenotype of the Arabidopsis ac15 mutant is suppressed by a mutation in an upstream ORF of a bHLH gene. (2006)
  • Author(s): Imai, A., Hanzawa, Y., Komura, M., Yamamoto, KT., Komeda, Y., Takahashi, T.
  • Journal Title: Development 133 Pages: 3575-3585
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of the class IV homeodomain-leucine zipper gene family in Arabidopsis. (2006)
  • Author(s): Nakamura, M., Katsumata, M., Abe, M., Yabe, N., Komeda, Y., Yamamoto, KT., Takahashi, T.
  • Journal Title: Plant Physiology 141 Pages: 1363-1375
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Metabolic changes in Arabidopsis thaliana expressing the feedback-resistant anthranilate synthase beta subunit gene OSASA1D. (2006)
  • Author(s): Ishihara, A., Asada, Y., Takahashi, Y., Yabe, N., Komeda, Y., Nishioka, T., Miyagawa, H., Wakasa, K.
  • Journal Title: Phytochemistry 67 Pages: 2349-2362
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Arabidopsis mutants by activation tagging in which photosynthesis genes are expressed in dedifferentiated calli. (2006)
  • Author(s): Niwa, Y., Goto, S., Nakano, T., Sakaiya, M., Hirano, T., Tsukaya, H., Komeda, Y., Kobayashi, H.
  • Journal Title: Plant and Cell Physiology 47 Pages: 319-331
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The dwarf phenotype of the Arabidopsis acl5 mutant is suppressed by a mutation in an upstream ORF of a bHLH gene (2006)
  • Author(s): Imai A, Hanzawa Y, Komura M, Yamamoto KT, Komeda Y, Takahashi T
  • Journal Title: Development 133 Pages: 3575-3585
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of the Class IV Homeodomain-Leucine Zipper Gene Family in Arabidopsis. (2006)
  • Author(s): Nakamura M, Katsumata M, Abe M, Yabe N, Komeda Y, Yamamoto KT, Takahashi T
  • Journal Title: Plant Physiol. 141 Pages: 1363-1375
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metabolic changes in Arabidopsis thaliana expressing the feedback-resistant anthranilate synthase beta subunit gene OASA1D (2006)
  • Author(s): Atsushi Ishihara, Yohei Asada, Yoshitaka Takahashi, Naoto Yabe, Yoshibumi Komeda, Takaaki Nishioka, Hisashi Miyagawa, Kyo Wakasa
  • Journal Title: Phytochemistry Volume 67, Issue 21 Pages: 2349-2362
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Arabidopsis Mutants by Activation Tagging in which Photosynthesis Genes are Expressed in Dedifferentiated Calli. (2006)
  • Author(s): Yasuo Niwa, Shingo Goto, Tatsuo Nakano, Mao Sakaiya, Takanori Hirano, Hirokazu Tsukaya, Yoshibumi Komeda, Hirokazu Kobayashi
  • Journal Title: Plant Cell Physiol. 47(3) Pages: 319-331
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of the class IV homeodomain-leucine zipper gene family in Arabidopsis. (2006)
  • Author(s): Nakamura, M. et al.
  • Journal Title: Plant Physiology 141・4 Pages: 1363-1375
• [Journal Article] The dwarf phenotype of the Arabidopsis acl5 mutant is suppressed by a mutation in an upstream ORF of a bHLH gene. (2006)
  • Author(s): Imai, A. et al.
  • Journal Title: Development 133・18 Pages: 3575-3585
• [Journal Article] 組換えDNA実験技術と植物遺伝学 (2006)
  • Author(s): 米田好文
  • Journal Title: 生物の科学・遺伝 60・1 Pages: 23-24
• [Journal Article] AtXTH27 plays an essential role in cell wall modification during the development of tracheary elements. (2005)
  • Author(s): Matsui, A., Yokoyama, R., Seki, M., Ito, T., Shinozaki, K., Takahashi, T., Komeda, y., Nishitani, K.
  • Journal Title: Plant Journal 42 Pages: 525-534
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] AtXTH27 plays an essential role in cell wall modification during the development of tracheary elements. (2005)
  • Author(s): Akihiro Matsui, Ryusuke Yokoyama, Motoaki Seki, Takuya Ito, Kazuo Shinozaki, Taku Takahashi, Yoshibumi Komeda, Kazuhiko Nishitani
  • Journal Title: Plant Journal 42 Pages: 525-534
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] AtXTH27 plays an essential role in cell wall modification during the development of tracheary elements. (2005)
  • Author(s): Matsui, A., -5-, Komeda, Y., Nishitani, K.
  • Journal Title: The Plant Journal 42・4 Pages: 525-534
• [Journal Article] Spermidine synthase genes are essential for survival of Arabidopsis. (2004)
  • Author(s): Imai, A., Matsuyama, T., Hanzawa, Y., Akiyama, T., Tamaoki, M., Saji, H., Shirano, Y., Kato, T., Hayashi, H., Shibata, D., Tabata, S., Komeda, Y., Takahashi, T.
  • Journal Title: Plant Physiology 135 Pages: 1565-1573
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Spermidine synthase genes are essential for survival of Arabidopsis. (2004)
  • Author(s): Imai A, Matsuyama T, Hanzawa Y, Akiyama T, Tamaoki M, Saji H, Shirano Y, Kato T, Hayashi H, Shibata D, Tabata S, Komeda Y, Takahashi T
  • Journal Title: Plant Physiol. 135 Pages: 1565-1573
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genetic Regulation of Time to Flower in Arabidopsis thaliana. (2004)
  • Author(s): Yoshibumi Komeda
  • Journal Title: Annual Review of Plant Biology. Volume 55 Pages: 521-535
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genetic regulation of time to flower in Arabidopsis thaliana. (2004)
  • Author(s): Komeda, Y.
  • Journal Title: Annual Review of Plant biology 55 Pages: 521-535
• [Journal Article] Flowering of Arabidopsis cop1 mutants in darkness. (2004)
  • Author(s): Nakagawa, M., Komeda, Y.
  • Journal Title: Plant and Cell Physiology 45・4 Pages: 398-406
• [Journal Article] The plant exon finder : a tool for precise detection of exons using a T-DNA-based tagging approach. (2004)
  • Author(s): Jingu F, Shirase T, Ohtomo I, Imai A, Komda Y, Takahashi T
  • Journal Title: Gene 338 Pages: 267-273
• [Journal Article] Spermidine synthase genes are essential for survival of Arabidopsis. (2004)
  • Author(s): Imai A, Matsuyama T, Hanzawa Y, Akiyama T, Tamaoki M, Saji H, Shirano Y, Kato Y, Hayashi H, Shibata D, Tabata S, Komeda, Y, Takahashi T.
  • Journal Title: Plant Physiology 135 Pages: 1565-1573
• [Journal Article] Metabolic changes in Arabidopsis thaliana expressing the feedback-resistant anthranilate synthase α subunit gene OASA1D.
  • Author(s): Ishihara, A. et al.
• [Book] Annual Review of Plant Biology (2004)
  • Author(s): Komeda, Y.
  • Publisher: Annual Review Inc.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Abe, M., Katsumata, H., Komeda, Y., Takahashi, T.: "Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis."Development. 130. 635-643 (2003)