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Evolution of reproductive traits and speciation process : approaches with experimental hybridization, genomic analysis and mathematical models

Research Project

Project/Area Number 15207004
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionKyoto University

Principal Investigator

SOTA Teiji  Kyoto University, Graduate School of Science, Associate Professor, 大学院理学研究科, 助教授 (00192625)

Co-Investigator(Kenkyū-buntansha) KUBOTA Kohei  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院農学生命科学研究科, 助教授 (30272438)
KAWATA Masakado  Tohoku University, Graduate School of Life Science, Professor, 大学院生命化学研究科, 教授 (90204734)
SASAKI Akira  Kyushu University, Graduate School of Science, Associate Professo, 大学院理学研究院, 助教授 (90211937)
SAWAMURA Kyoichi  University of Tsukuba, Graduate School of Life and Environmental Sciences, lecturer, 大学院生命環境科学研究科, 講師 (90247205)
河田 雅圭  東北大学, 大学院・生命科学研究科, 教授 (11301945)
佐々木 顕  九州大学, 大学院・理学研究院, 助教授 (17102884)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥33,670,000 (Direct Cost: ¥25,900,000、Indirect Cost: ¥7,770,000)
Fiscal Year 2006: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2005: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2004: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2003: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Keywordsspeciation / reproductive isolation / genitalia / quantitative genetics / linkage map / sperm competition / cuticular hydrocarbon / introgressive hybridization / AFLP / マイクロサテライト / QTL / 遺伝子系統樹 / 性フェロモン / 繁殖形質 / 交雑 / 遺伝的基盤 / 遺伝的多型 / 分子系統
Research Abstract

In this study, we have focused on a ground beetle group Ohomopterus in Japan, which consists of 15 species with marked interspecific diversity of genital morphology. We analyzed the genetic basis of interspecific difference in genital morphology between two Ohomopterus ground beetles using a quantitative genetic and QTL mapping approach. In the quantitative genetic analysis, it was inferred that relatively small number of genes produced the large difference in genitalia between 0. iwawakianus and 0. maiyasanus. We also conducted a QTL mapping with F2 hybrids of these species using AFLP and microsatellite markers. We analyzed evolutionary trend of genital morphology in relation to other reproductive traits using a comparative method, and found that exaggeration of genital morphology occurred in association with spermatophore size and copulation time. In addition, we analyzed shape of sperm and sperm-bundles which can be selected for improvement of sperm-competition ability. We found a d … More imorphism of sperm-bundles, i.e. long and short types, in most species and revealed that long sperm bundles have been evolved in association with other reproductive traits including genital parts. The evolution of genital morphology is considered to have occurred within populations through sexual selection and could have contributed to mechanical reproductive isolation which promotes speciation. A similar effect is expected for differentiation in body size. Using interspecific mitochondrial gene flow as an index of intrgressive hybridization, we revealed that differences in genital and body sizes had effect to prevent introgressive hybridization. Besides the above studies, we also studied the effect of cuticular hydrocarbon (CHC) on mate recognition and variation in CHC among species to explore the potential role of CHC as sexual pheromones to enable coexistence of closely related species. In addition, the pattern and strength of pre-and post-mating reproductive isolation between various pairs of Ohomopterus species. Less

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (34 results)

All 2007 2006 2005 Other

All Journal Article (33 results) Publications (1 results)

  • [Journal Article] Asymmetry in reproductive isolation and its effect on directional mitochondrial introgression in the parapatric ground beetles Carabus yamato and C. alhrechti (Coleoptera, Carabidae).2007

    • Author(s)
      Takami, Y., N.Nagata, M.Sasabe, T.Sota
    • Journal Title

      Population Ecology (印刷中)(未定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rapid diversification of male genitalia and mating strategies in Ohomopterus ground beetles2007

    • Author(s)
      Takami, Y., T. Sota
    • Journal Title

      Journal of Evolutionary Biology (印刷中)(未定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The genetic basis of interspecific differences in genital morphology of closely related carabid beetles2007

    • Author(s)
      Sasabe, M., Y.Takami, T.Sota
    • Journal Title

      Heredity 98

      Pages: 385-391

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phylogeography and introgressive hybridization of the ground beetle Carabus yamato in Japan based on mitochondrial gene sequences2007

    • Author(s)
      Nagata, N., K.Kubota, T.Sota
    • Journal Title

      Zoological Science 24

      Pages: 465-474

    • NAID

      120002056254

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The role of cuticular hydrocarbons in mating and conspecific recognition in closely related longicorn beetles Pidonia grallatrix and P. takechii2007

    • Author(s)
      Tanigaki, T., R.Yamaoka, T.Sota
    • Journal Title

      Zoological Science 24

      Pages: 39-45

    • NAID

      10018877901

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sperm competition promotes diversity of sperm bundles in Ohomopterus ground beetles2007

    • Author(s)
      Takami, Y., T. Sota
    • Journal Title

      Naturwissenschaften (印刷中)(未定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The role of cuticular hydrocarbons in mating and conspecific recognition in closely related longicorn beetles Pidonia grallatrix and P. takechii.2007

    • Author(s)
      Tanigaki, T., Yamaoka, R., T.Sota
    • Journal Title

      Zoological Science 24

      Pages: 39-45

    • NAID

      10018877901

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The genetic basis of interspecific differences in genital morphology of closely related carabid beetles.2007

    • Author(s)
      Sasabe, M., Takami, Y., Sota, T.
    • Journal Title

      Heredity 98

      Pages: 385-391

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phylogeography and introgressive hybridization of the ground beetle Carabus yamato in Japan based on mitochondrial gene sequences.2007

    • Author(s)
      Nagata, N., Kubota, K., T.Sota, T.
    • Journal Title

      Zoological Science 24

      Pages: 465-474

    • NAID

      120002056254

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rapid diversification of male genitalia and mating strategies in Ohomopterus ground beetles.2007

    • Author(s)
      Takami, Y., Sota, T.
    • Journal Title

      Journal of Evolutionary Biology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Sperm competition promotes diversity of sperm bundles in Ohomopterus ground beetles.2007

    • Author(s)
      Takami, Y., Sota, T.
    • Journal Title

      Naturwissenschaften (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Asymmetry in reproductive isolation and its effect on directional mitochondrial introgression in the parapatric ground beetles Carabus yamato and C. albrechti (Coleoptera, Carabidae).2007

    • Author(s)
      Takami, Y., Nagata, N., Sasabe, M., Sota, T.
    • Journal Title

      Population Ecology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The genetic basis of interspecific differences in genital morphology of closely related carabid beetles2007

    • Author(s)
      Sasabe M, Takami Y, Sota T
    • Journal Title

      Heredity (印刷中)(未定)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sperm competition promotes diversity of sperm bundles in Ohomopterus ground beetles2007

    • Author(s)
      Takami Y, Sota T
    • Journal Title

      Naturwissenchaften (印刷中)(未定)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rapid diversification of male genitalia and mating strategies in Ohomopterus ground beetles2007

    • Author(s)
      Takami Y, Sota T
    • Journal Title

      Journal of Evolutionary Biology (印刷中)(未定)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Phylogeography and introgressive hybridization of the ground beetle Carabus yamato in Japan based on mitochondrial gene sequences2007

    • Author(s)
      Nagata N, Kubota K, Sota T
    • Journal Title

      Zoological Science (印刷中)(未定)

    • NAID

      120002056254

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Adaptive divergence of scaling relationships mediates the arms race between a weevil and its host plant2006

    • Author(s)
      Toju, H., T.Sota
    • Journal Title

      Biology Letters 2

      Pages: 539-542

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Utility of nuclear allele networks for the analysis of closely-related Species in the genus Carabus, subgenus Ohomopterus2006

    • Author(s)
      Sota, T., M.Sasabe
    • Journal Title

      Systematic Biology 55

      Pages: 329-344

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genital lock-and-key system and premating isolation by mate preference in carabid beetles (Carabus subgenus Ohomopterus)2006

    • Author(s)
      Usami, T., Yokoyama, J., Kubota, K., Kawata, M
    • Journal Title

      Biological Journal of the Linnean Society 87

      Pages: 145-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Four new species of the Australian carabid beetles Pamborus Latreille (Coleoptera, Carabidae)2006

    • Author(s)
      Takami, Y., T.Sota, T.
    • Journal Title

      Australian Journal of Entomology 45

      Pages: 44-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Utility of nuclear allele networks for the analysis of closely-related Species in the genus Carabus, subgenus Ohomopterus.2006

    • Author(s)
      Sota, T., Sasabe, M.
    • Journal Title

      Systematic Biology 55

      Pages: 329-344

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Four new species of the Australian carabid beetles Pamborus Latreille (Coleoptera, Carabidae).2006

    • Author(s)
      Takami, Y., T.Sota, T.
    • Journal Title

      Australian Journal of Entomology 45

      Pages: 44-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genital lock-and-key system and premating isolation by mate preference in carabid beetles (Carabus subgenus Ohomopterus).2006

    • Author(s)
      Usami, T., Yokoyama, J., Kubota, K., Kawata, M.
    • Journal Title

      Biological Journal of the Linnean Society 87

      Pages: 145-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adaptive divergence of scaling relationships mediates the arms race between a weevil and its host plant.2006

    • Author(s)
      Toju, H., Sota, T.
    • Journal Title

      Biology Letters 2

      Pages: 539-542

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Utility of allele networks for the analysis of closely related species in the genus Carabus, subgenus Ohomopterus2006

    • Author(s)
      Sota T, Sasabe M
    • Journal Title

      Systematic Biology 55(2)

      Pages: 329-344

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Adaptive divergence of scaling relationships mediates the arms race between a weevil and its host plant2006

    • Author(s)
      Toju H, Sota T
    • Journal Title

      Biology Letters 2

      Pages: 539-542

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Utility of nuclear allele networks for the analysis of closely-related Species in the genus Carabus, subgenus Ohomopterus2006

    • Author(s)
      Sota, T., M.Sasabe
    • Journal Title

      Systematic Biology (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genital lock-and-key system and premating isolation by mate preference in carabid beetles (Carabus subgenus Ohomopterus)2006

    • Author(s)
      Usami, T., J.Yokoyama, K.Kubota, M.Kawata
    • Journal Title

      Biological Journal of the Linnean Society 87

      Pages: 145-154

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Parallel formation of hybrid swarms of ground beetles in the genus Carabus (Coleoptera : Carabidae) in adjacent river basins2005

    • Author(s)
      Ujiie, M., K.Kubota, T.Sota, R.Ishikawa
    • Journal Title

      Entomological Science 8

      Pages: 429-437

    • NAID

      10017170932

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phylogeny and character evolution of endemic Australian carabid beetles of the genus Pamborus based on mitochondrial and nuclear gene sequences2005

    • Author(s)
      Sota, T., Y.Takami, G.B.Monteith, B.P.Moore
    • Journal Title

      Molecular Phylogenetics and Evolution 36

      Pages: 391-404

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phylogeny and character evolution of endemic Australian carabid beetles of the genus Pamborus based on mitochondrial and nuclear gene sequences.2005

    • Author(s)
      Sota, T., Takami, Y., Monteith, G.B., Moore B.P.
    • Journal Title

      Molecular Phylogenetics and Evolution 36

      Pages: 391-404

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Parallel formation of hybrid swarms of ground beetles in the genus Carabus (Coleoptera : Carabidae) in adjacent river basins.2005

    • Author(s)
      Ujiie, M., Kubota, K., Sota, T., Ishikawa, R.
    • Journal Title

      Entomological Science 8

      Pages: 429-437

    • NAID

      10017170932

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phylogeny and character evolution of endemic Australian carabid beetles of the genus Pamborus based on mitochondrial and nuclear gene sequences2005

    • Author(s)
      Sota, T., Takami, Y., Monteith, G.B., Moore, B.P.
    • Journal Title

      Molecular Phylogenetics and Evolution (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Publications] Sota, T., R.Ishikawa: "Phylogeny and life-history evolution in Carabus (subtribe Carabina : Coleoptera, Carabidae) based on sequences of two nuclear genes"Biological Journal of the Linnean Society. 81(1). 135-149 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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