| Project/Area Number |
15208003
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Horticulture/Landscape architecture
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
GEMMA Hiroshi University of Tsukuba, Graduate school of Life and Environmental Science, Professor, 大学院・生命環境科学研究科, 教授 (70094406)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
EZURA Hiroshi University of Tsukuba, Graduate school of Life and Environmental Science, Professor, 大学院・生命環境科学研究科, 教授 (00332552)
SUGAYA Sumiko University of Tsukuba, Graduate school of Life and Environmental Science, Assistant professor, 大学院・生命環境科学研究科, 講師 (90302372)
ITAMURA Hiroyuki Shimane university, Faculty of Life and Environmental Science, Professor, 生物資源科学部, 教授 (80109040)
NAKAGAWA Tsuyoshi Shimane university, Integrated science research supporting center, Associate professor, 総合科学研究支援センター(遺伝子機能開発分野), 助教授 (30202211)
NAKATSUKA Akira Shimane university, Faculty of Life and Environmental Science, Associate professor, 生物資源科学部, 助教授 (40304258)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2005: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2004: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2003: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
|
|---|
| Keywords | Persimmon / Ethylene / Ethylene synthesis enzyme genes / Ethylene receptor enzyme genes / softening / deastringency / cell wall degradation enzymes / real time PCR / カキ果実 / ナシ果実 / Race-PCR |
|---|
| Research Abstract |
This study was carried out to elucidate of ethylene signal transduction mechanism and its regulation on fruit maturation, ripening and rapid softening as a physiological disorder after deastringency, using several cultivars of persimmon. Irrespective of cultivars, it is clear that ethylene promotes the softening because 1-MCP treatment depressed the cell wall degradation enzymes activities and their encoding genes such as β-galactosidase (DKB-gal) and xyloglucan endotransglycosylase (DKXTH1). Similarly, DKXTH1 was induced by alcohol treatment on 'Saijo' young fruit. On the contrary, it was found on 'Saijo' after deastringency treatment that xylosidase/afrbinofranosidase genes (DKARF1 and 2) expression was promoted.
Expression of ethylene biosynthesis enzymes genes were analyzed throughout the fruit development and maturation of both cultivars ; 'Saijo' and 'Rendaiji', As a result, there were difference in strongly expressed genes between young and ripened fruits ; DKACS2 and DKACS3 as A
… More
CC synthesis enzyme genes were prominent in young 'Saijo' and 'Rendaiji' fruits, respectively. By contrast in ripened fruit, DKACS1 was supposed to be a major gene to function in parallel with DKACO1 and DKACO2 expression as ACC oxidation enzyme genes in both cultivars.
Three full-length persimmon cDNAs homologous to Arabidopsis ethylene receptor genes ERS1, ETR1, and ETR2 were isolated. Subsequently, DkERS1, DkETR1, and DkETR2 were designated, respectively, and examined their expression during fruit development and ripening. DkETR1 mRNA expression remained at a basal level throughout all stages examined and was not affected by ethylene treatment. In contrast, expression of DkERS1 and DkETR2 mRNAs was correlated with ethylene production during fruit development and ripening and was enhanced after ethylene treatment. Western blot analysis using anti-DkERS1 antibody showed that expression of DkERS1 protein degreased gradually towards maturation and reached the lowest level at the ripening stage, in agreement with the ethylene receptor-inhibition model. These results imply that DkERS1 may play a major role in sensing ethylene signals associated with fruit development and ripening. Less
|
